Biolegend, 767302, Recombinant Human/Mouse/Rat BMP-2 (carrier-free)

Name: Recombinant Human/Mouse/Rat BMP-2 (carrier-free)
Brand: Biolegend
SKU: 767302

10microg

Catalog Number: 767302

Pricing

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Category

Recombinant Growth Factors

Description

Bone morphogenetic protein 2 (BMP-2) belongs to the TGF-β superfamily. Like other members of BMP family, BMP-2 is synthesized as an inactive propeptide precursor which dimerizes and then it is further processed into mature form by proprotein convertases (PCs). Some evidence indicated that PC5/6A and Factor VII-activating protease (FSAP) are involved in maturation of BMP-2. Mature BMP-2 is a 26 kD protein composed of 114 amino acids, forming three intramolecular and one intermolecular disulfide bond. BMP-2 forms homodimer or heterodimer with other BMP proteins, including BMP-4, BMP-6 and BMP-7. BMP-2 signal through heterodimeric serine/threonine kinase receptors composed of a type I (BMPR1a/ALK3, BMPR1b/ALK6, ActRIa/ALK2) and a type II (BMPR2, ACVR2a/ActRIIA, ACVR2b/ActRIIB). BMP-2 binds to the type I receptor with high affinity, in turn recruits the type II receptor. BMP-2 stimulation initiates receptor shutting, leading to receptor clustering and activation of the downstream signaling. BMP-2 signals via canonical Smad or other downstream kinase, such as p38 and JNK in a context-dependent manner. BMP-2 is involved in several processes, including cartilage and bone formation, differentiation, and embryogenesis. BMP-2 induces osteogenic differentiation in human mesenchymal stem cells and myogenic cells. BMP-2 induces cartilage repair and remodeling by stimulating chondrocyte proliferation and expression of matrix proteins. BMP-2/BMP-7 heterodimer is more potent in the induction of bone formation in vivo than BMP-2 homodimer. BMP-2-deficiency leads to embryonic lethality with abnormal cardiac development, malformation of the amnion/chorion, severe chondrodysplasia, and defects in myocardial patterning, suggesting that BMP-2 mediates organ morphogenesis. Noggin is an antagonist to reverse BMP-2-mediated effect and its expression is induced by BMP-2 in osteoblasts as a negative feedback loop. In addition, BMP-2 stimulates epithelial to mesenchymal cell transformation through TGF-β type III receptor.

Specifications

General

Source	Human/Mouse/Rat BMP-2, amino acid Ala284-Arg396 (Accession# P12643), was expressed in E. coli.
Molecular Mass	The 113 amino acid recombinant protein has a predicted molecular mass of approximately 12.8 kD. The DTT-reduced and non-reduced protein migrates at approximately 13 and 26 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Ala.
Purity	> 98%, as determined by Coomassie stained SDS-PAGE.
Formulation	Sterile-filtered with 0.2 µm filter, solution comprised of 4 mM HCl.
Endotoxin Level	Less than 0.1 EU/µg cytokine as determined by the LAL method.
Concentration	10-25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes and larger are bottled at the concentration indicated on the vial.
Storage & Handling	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Ordering

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Biolegend, 767302, Recombinant Human/Mouse/Rat BMP-2 (carrier-free)

Send an RFQ.

General

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