Biolegend, 768914, Biotinylated Recombinant Human FAP (carrier-free)
25microg
Catalog Number: 768914
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| Source | Human FAP, amino acids Leu26-Asp760 (Accession # Q12884), with a C-terminal 6His tag and Avi tag was expressed in CHO cells. Human FAP was site-specifically biotinylated by BirA. |
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| Molecular Mass | The 761 amino acid recombinant protein has a predicted molecular mass of approximately 87.9 kD. The DTT-reduced and non-reduced protein migrate at approximately 80-90 kD in SDS-PAGE. |
| N-terminalSequence Analysis | The predicted N-terminal amino acid is Leu. |
| Purity | > 95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.22 µm filtered protein solution is in 16 mM Tris, 240 mM NaCl, 20% Glycerol, pH 7.5 |
| Endotoxin Level | Less than 0.1 EU per μg of protein as determined by the LAL method |
| Concentration | 25 µg and 100 µg at 1.0 mg/mL |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at - 20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
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| Activity | Biotinylated human FAP cleaves a fluorogenic substrate Z-Gly-Pro-AMC with a specific activity value > 1800 pmol/µg/min. |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Monomer |
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| Distribution | Adipose tissue and Cancer associated fibroblasts (CAFs) |
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| Function | Tissue remodeling, fibrosis, wound healing, inflammation, tumor growth and metastasis |
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| Interaction | Serpin F2 (alpha-2-antiplasmin); Gelatin (Type I collagen) |
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| Bioactivity | Biotinylated human FAP cleaves a fluorogenic substrate Z-Gly-Pro-AMC with a specific activity value > 1800 pmol/µg/min. |
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| Biology Area | Cancer Biomarkers |
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| Molecular Family | Enzymes and Regulators |
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| Antigen References | Ostermann E. et al. 2008. Clin Cancer Res.14:4584. Kakarla S. et al. 2013. Mol Ther. 21:1611. Gulati P. et al. 2018. Clin Cancer Res. 24:3981. Freedman JD. et al. 2018. Cancer Res. 78: 6852. Schuberth PC. et al. 2013. J Transl. Med. 11:187 Garin‐Chesa P. et al. 1990. Proc Natl Acad Sci USA. 87:7235. Nurmik M. et al. 2020. Int J Cancer. 146:895. Wang W. et al. 2025. J Control Release. 384:113888 |
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| Gene ID | 2191 |
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| UniProt | View information about FAP on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | FAP, FAP-alpha, Fibroblast activation protein alpha, Integral membrane serine protease, Seprase, DPP4 |
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