Biolegend, 770204, Biotinylated Recombinant Human CD200-Fc Chimera (carrier-free)
25microg
Catalog Number: 770204
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| Source | Human CD200, amino acids Gln31-Gly232 (Accession # P41217) with a C-terminal human IgG1 Fc and 6-His tag was expressed in 293E cells. |
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| Molecular Mass | The unlabeled 446 amino acid recombinant protein has a predicted molecular mass of approximately 50 kD. The DTT-reduced protein migrates at approximately 65 kD and and non-reduced protein migrates at approximately 130 kD by SDS-PAGE. The predicted N-terminal amino acid is Gln. |
| Purity | >95 %, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.22 µm filtered protein solution is in PBS, pH 7.2, 5% Glycerol |
| Endotoxin Level | Less than 1.0 EU per µg protein as determined by the LAL method. |
| Concentration | 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Storage & Handling |
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| Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Activity | When human CD200R1 is immobilized at 0.5 µg/mL, human CD200 Biotin binds with EC50 of 1.5 – 6.0 ng/mL. |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Disulfide-linked homodimer, biotinylated via amines |
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| Distribution | Neurons and vascular endothelium in CNS, and most peripheral cell types, thymocytes, T and B cells, and dendritic cells. |
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| Function | Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues. |
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| Interaction | Mast cells, basophils, macrophages, and dendritic cells |
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| Ligand/Receptor | CD200R |
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| Bioactivity | Measured by it’s ability to bind immobilized hCD200R1 |
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| Biology Area | Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | CD Molecules, Soluble Receptors |
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| Antigen References | 1. Hernangomez M, C. et al. 2014. Curr Pharm Des. 20:4707-22. 2. Walker DG, Lue LF. 2013. Future Neurol. 10.2217/fnl.13.14. 3. Wright GJ, et al. 2003. J Immunol. 171: 3034-46. 4. Vaine CA, Soberman RJ. 2014. Adv Immunol. 121:191-211. 5. Minas K, et al. 2006. Crit Rev Immunol. 26(3): 213-30. 6. Wang XJ, et al. 2007. J Neuroimmune Pharmacol. 2(3): 259-64. 7. Wong KK, et al. 2010. J Leukoc Biol. 88(2): 361-72. |
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| Gene ID | 4345 |
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| UniProt | View information about CD200 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | OX-2 membrane glycoprotein, OX-2, OX2, MRC OX-2, MOX1, MOX2, My033 |
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