Biolegend, 771104, Recombinant Mouse GM-CSFRα-Fc Chimera (carrier-free)
25microg
Catalog Number: 771104
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| Source | Mouse GM-CSFRα, amino acid Leu30 – Pro327 (Accession # Q00941) with a C-terminal human IgG Fc - 6His tag, was expressed in 293E cells. |
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| Molecular Mass | The 542 amino acid recombinant protein has a predicted molecular mass of approximately 59.5 kD. The DTT-reduced and non-reduced protein migrates at approximately 75 and 150kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Leu. |
| Purity | >98%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | Sterile-filtered with 0.2 µm filter, solution comprised of PBS pH 7.2. |
| Endotoxin Level | < 0.1 EU/µg cytokine as determined by the LAL method |
| Concentration | 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
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| Activity | The activity was measured by functional ELISA. Immobilized recombinant mouse GM-CSFRα, 100 µl at 2 µg/mL, binds recombinant mouse GM-CSF (Cat. No. 576302) in a dose dependent manner. The ED50= 3-15 ng/ml. |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Cytokine |
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| Distribution | Monocytes, granulocytes, tissue macrophages, type II alveolar epithelial cells, CD34+ progenitor cells, uterine cells, vascular endothelial cells, and fibroblasts. |
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| Function | Modulate effector functions on myeloid cells, type II alveolar epithelial cells, CD34+ progenitor cells, uterine cells, vascular endothelial cells, and fibroblasts. Toll-like receptor agonists induce its expression in RAW 264.7 cells. |
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| Interaction | β-chain (βc) |
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| Ligand/Receptor | GM-CSF |
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| Bioactivity | Soluble GM-CSFRα antagonizes GM-CSF binding and function. |
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| Biology Area | Stem Cells |
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| Molecular Family | Cytokine/Chemokine Receptors, Soluble Receptors |
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| Antigen References | Park LS, et al. 1998. Proc Natl Acad Sci U S A. 89:4295. Prevost GM, et al. 2002. J Immunol. 169:5679. Hamilton JA, Anderson GP. 2004. Growth Factors. 22:225. Rosas M, et al. 2007. Eur J Immunol. 37:2518. Kared H, et al. 2008. Blood. 112:2575. Bhattacharya P, et al. 2011. J Leukoc Biol. 89:235. Bhattacharya P, et al. 2015. Cytokine. 75:261. |
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| Gene ID | 12982 |
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| UniProt | View information about GM-CSFRalpha on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | CD116, Colony Stimulating Factor 2 Receptor Alpha Subunit, Csfgmra, GM-CSFR, GM-CSF-Ra |
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