Biolegend, 777215, Biotinylated Recombinant Human CD40L (TNFSF5) (carrier-free)
50microg
Catalog Number: 777215
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Category
Pack Size40 L
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| Source | Human CD40L, amino acids Gly116-Leu261 (Accession# P29965) was expressed in 293E cells. The N-terminal contains an Avi-8His tag. Human CD40L-Avi tag was site-specifically biotinylated by enzyme BirA. |
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| Molecular Mass | The 192 amino acid recombinant protein has a predicted molecular mass of approximately 20.6 kD. The protein migrates at approximately 27 - 30 kD in DTT-reducing and non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Gly. |
| Purity | > 95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.22 µm filtered protein solution is in PBS, 5% Trehalose, pH 7.2 |
| Endotoxin Level | Less than 0.1 EU per µg cytokine as determined by the LAL method |
| Concentration | 50 µg size lyophilized |
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| Storage & Handling |
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| Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C or colder until the expiration date. Reconstitute lyophilized protein in sterile PBS, pH7.2. Before reconstitution, make sure sterile PBS and product are at room temperature. Quickly spin the vial or gently tap down on the vial to make sure the lyophilized product is at the bottom of the vial before opening. Use aseptic techniques to add the required volume of reconstitution buffer (sterile PBS) to the vial, to obtain the recommended stock concentration 250 µg/mL. Close the vial and leave at an ambient temperature for 15-20 minutes. Then gently invert the vial several times or until all the lyophilized product dissolves. Leave the vial at room temperature for another 15 minutes. If small particulates are still observed after 15 minutes, incubate at room temperature for an additional 30 minutes, and leave the vial at 2°C - 8°C overnight. Next day, invert the vial several times or gently pipette the solution up and down before use. If needed, transfer the reconstituted stock solution to a sterile container for additional dilution to no less than 100 µg/mL. Small working aliquots in polypropylene tubes can be made after reconstitution and store the vials at -20°C or lower. Avoid freeze/thaw cycles. Carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to two weeks or stored at -20°C or colder for up to 3 months. |
| Activity | Biotinylated recombinant human CD40L binds to immobilized recombinant human CD40-Fc chimera (Cat. No. 777204) in a dose-dependent manner. The ED50 for this effect is 0.6 – 4.0 ng/mL. |
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| Application | Bioassay |
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| Application Notes | Our lyophilized proteins are validated in-house to maintain activity after shipping at ambient temperature and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Homotrimer |
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| Distribution | T cells, basophils, mast cells, activated platelets |
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| Function | CD40L induces proliferation, differentiation, and immunoglobulin class switching in B cells; also prevents apoptosis in B cells. CD40L is induced in T cells after in vitro activation with PHA, PMA plus ionomycin, anti-CD3, anti-CD2, and CD28 engagement in anti-CD3 activation. Also, CD40L is induced by thrombin in platelets. |
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| Interaction | B cells, neutrophils, follicular dendritic cells, dendritic cells, macrophages, epithelial cells, epithelial carcinoma cells, endothelial cells, smooth muscle cells, fibroblasts, hepatic stellate cells, and glomerular mesangial cells |
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| Ligand/Receptor | CD40 |
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| Bioactivity | Biotinylated recombinant Human CD40L binds to immobilized recombinant human CD40. |
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| Biology Area | Cell Biology, Costimulatory Molecules |
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| Molecular Family | Cytokines/Chemokines, Growth Factors, Immune Checkpoint Receptors |
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| Antigen References | Spriggs MK, et al. 1992. J. Exp. Med. 176:1543. Mazzei GJ, et al. 1995. J. Biol. Chem. 270:7025. Delmas Y, et al. 2005. Med. Sci. (Paris). 21:825. Meabed MH, et al. 2007. Haematology. 12:301. Sprangue DL, et al. 2008. Blood. 111:5028. Davidson DC, et al. 2012. PLoS One. 7: e51793. Rahman M, et al. 2013. J. Thromb. Haemost. 11:1385. |
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| Gene ID | 959 |
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| UniProt | View information about CD40L on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | CD154, TNF-related activator protein (TRAP), GP39, TNFSF5 |
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