Biolegend, 782903, Recombinant Porcine CXCL8/IL-8 (carrier-free)
20microg
Catalog Number: 782903
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| Source | Porcine CXCL8, amino acid Ala26 – Gln103 (Accession #P26894) was expressed in E.coli. |
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| Molecular Mass | The 78 amino acid recombinant protein has a predicted molecular mass of approximately 9.0 kD. The predicted N-terminal amino acid is Ala. |
| Purity | >95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | Lyophilize from sterile 0.22 µm filtered protein solution is in PBS pH 7.2. |
| Endotoxin Level | Less than 1 EU per µg protein as determined by the LAL method. |
| Concentration | Lyophilized recombinant protein is at 5 and 20 µg total protein |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Reconstitute the protein in sterile water at 0.1 mg/mL. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles. |
| Activity | Recombinant Porcine CXCL8/IL-8 chemoattracts human neutrophils in a dose-dependent manner. The ED50 for this effect is = 2- 10 ng/mL |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in lliophylized format are shipped on blue ice. |
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| Structure | Chemokine |
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| Distribution | Macrophages, epithelial cells, airway smooth muscle cells, and endothelial cells. |
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| Function | Chemoattraction and activation of neutrophils and granulocytes in inflammation. Induced by inflammatory cytokines such as TNFa, IL-1a; also induced by LPS |
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| Interaction | Neutrophils, monocytes/macrophages, T and NK cells, mast cells, and endothelial cells. |
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| Ligand/Receptor | CXCR1, CXCR2 |
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| Bioactivity | Recombinant Porcine CXCL8/IL-8 chemoattracts human neutrophils in a dose-dependent manner. |
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| Cell Type | Th17 |
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| Biology Area | Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | Burrows SD, et al. 1994. Biochemistry. 33:12741. Hoffmann E, et al. 2002. J Leukoc Biol. 2002 72:847. Araki S, et al. 2007. Cancer Res. 67: 6854. Ravindran A, et al. 2009. Biochemistry. 48:8795. Das ST, et al. 2010. PLoS One. 5:e11754. Baggiolini M. 2015. Front Immunol. 6:285. Liu Q, et al. 2016. Cytokine Growth Factor Rev. 31:61. |
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| Gene ID | 396880 |
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| UniProt | View information about CXCL8 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Small inducible cytokine subfamily B, member 8 (SCYB8), Monocyte derived neutrophils chemotactic factor (MDNCF), Neutrophil-activating peptide 1 (NAP1), Granulocyte chemotactic protein 1 (GCP1), Chemokine, CXC motif, ligand 8 |
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