Biolegend, 786704, Biotinylated Recombinant Human CTLA-4-Fc Chimera (carrier-free)

Name: Biotinylated Recombinant Human CTLA-4-Fc Chimera (carrier-free)
Brand: Biolegend
SKU: 786704

25microg

Catalog Number: 786704

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Category

Amino Acids

Description

Human cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) is a member of the IgG superfamily. It encodes a 223 amino acid protein and possesses a single extracellular V domain, a transmembrane domain, and a cytoplasmic domain. CTLA-4 is ~30% homologous with CD28, and both bind to the same ligands (B7-1/CD80 and B7-2/CD86) although CTLA-4 binds with higher affinity than CD28. Parallel recognition of a specific MHC-peptide complex by the T-cell receptor and of CD80 or CD86 by the costimulatory receptor CD28 results in T cell activation, cytokine production, proliferation, and differentiation. After the T cells' activation process and upregulation of CTLA-4, co-ligation of the TCR to the MHC-peptide complex and CTLA-4 to its ligands results in cell cycle arrest and ends T cell activation. It has been suggested that the negative regulatory mechanism of CTLA-4 is mediated through trans-endocytosis of CD80 and CD86 (capture of ligands from opposing cells). The ligands are removed from the original cell and degraded inside of CTLA-4-expressing cells. As a result, the action of CD28 is impaired. Alternative splice variants of CTLA-4 have been described in mice and humans that have transcripts that skip exon 2 (ligand-binding domain) or exon 3 (transmembrane domain). The ligand independent CTLA-4 is expressed in mice, but not in humans, and soluble CTLA-4 (sCTLA-4) is expressed in mice and humans. CTLA-4 is constitutively expressed by Tregs, and they are an important source of sCTLA-4. It has been suggested that sCTLA-4 potentiates regulatory T cell function. High levels of sCTLA-4 in Tregs have been associated with type 1 diabetes (T1D) and other autoimmune diseases, such as Grave's disease and myasthenia gravis. Also, CTLA-4 has been linked with proliferation and survival of chronic lymphocytic leukemia. CTLA-4 is a target for therapeutic intervention; in fact, a monoclonal antibody against CTLA-4 (Ipilimumab) has been approved for the treatment of melanoma.

Specifications

General

Source	Human CTLA-4, amino acids Ala31-Phe162 (Accession # BC070162) with a C-terminal human IgG1 Fc tag was expressed in CHO cells.
Molecular Mass	The unlabeled 360 amino acid recombinant protein has a predicted molecular mass of approximately 39.9 kD. The DTT-reduced and non-reduced glycosylated protein migrate at approximately 56 kD and 105 kD respectively by SDS-PAGE. The N-terminal amino acid is Ala.
Purity	>95%, as determined by Coomassie stained SDS-PAGE.
Formulation	0.22 µm filtered protein solution is in PBS, pH 7.2, 5% glycerol.
Endotoxin Level	Less than 0.01 ng/µg cytokine as determined by the LAL method.
Concentration	10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling	Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 0.2 - 1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

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Biolegend, 786704, Biotinylated Recombinant Human CTLA-4-Fc Chimera (carrier-free)

Send an RFQ.

General

Notice

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