Biolegend, 794604, Recombinant Human CES1 (carrier-free)
25microg
Catalog Number: 794604
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| Source | Human CES1, amino acid (His19 – Glu563) (Accession # AB119996.1) was expressed in CHO cells. The C-terminus contains a 6His-GGQ tag. |
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| Molecular Mass | The 558 amino acid recombinant protein has a predicted molecular mass of approximately 61.5 kD. The DTT-reduced and non reduced protein migrates at approximately 70 kD and 60 - 80 kD respectively by SDS-PAGE. The C-terminus contains a 6His-GGQ tag. The predicted N-terminal amino acid is His. |
| Purity | > 95%, as determined by Coomassie stained SDS-PAGE |
| Formulation | 0.22 µm filtered protein solution is in 50 mM Sodium Acetate, 150 mM NaCl, pH 5.0. |
| Endotoxin Level | Less than 0.1 EU per µg cytokine as determined by the LAL method |
| Concentration | 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
| Storage & Handling | Unopened vial can be stored at -20°C or -70°C for six months. For maximum results, quick spin vial prior to opening. Avoid repeated freeze/thaw cycles. |
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| Activity |
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| Human carboxylesterase 1 (CES1) hydrolyzes the compound, 4-nitrophenyl acetate in a colorimetric assay. The specific activity is > 6,000 pmol/min/µg in the presence of 0.04 µg of recombinant human CES1. |
| Application | Bioassay |
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| Application Notes | Human CES1 Enzymatic Assay Human carboxylesterase 1 hydrolyzes the carboxylic acid substrate, 4-nitrophenyl acetate. The increase of hydrolyzed product is monitored by an increase in absorbance at 400 nm. The specific activity is > 6,000 pmol/min/µg in the presence of 0.04 µg of recombinant human CES1. Materials Assay buffer: 50 mM Tris, pH 7.5 Recombinant human CES1 Substrate: 4-nitrophenyl acetate (4-NPA) (Sigma, Cat. No. N-8130), 100 mM stock in acetone 96-well clear plate (Costar, Cat. No. 92592) Plate reader (Model: SpectraMax Plus by Molecular Devices or equivalent) Procedure Dilute the recombinant human CES1 to 0.8 µg/mL in assay buffer. Dilute the substrate to 2 mM in deionized water. In a plate load 50 μL of human CES1 to the wells and start the reaction by adding 50 µL of 2 mM substrate. Include a substrate blank containing 50 µL of substrate and 50 µL assay buffer. Read at a wavelength of 400 nm (bottom read) in kinetic mode for 5 minutes. Calculate specific activity: Specific Activity (pmol/min/µg) = Adjusted Vmax* (OD/min) x Conversion Factor** (pmol/OD) amount of enzyme (µg) *Adjusted for substrate blank **Derived using calibration standard 4-Nitrophenol (Sigma, Cat. No. 241326) Per Well: Recombinant human CES1: 0.04 µg Substrate: 1 mM BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Homotrimer |
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| Distribution | Liver, human lung, and in monocytes/macrophages, absent in intestine |
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| Function | Drug clearance, activation, and deactivation. Hydrolysis of exogenous and endogenous compounds such as carboxylic acid esters, thioesters, carbamates, and amides. |
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| Interaction | Xenobiotics and fatty acids, cholesterol |
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| Bioactivity | Human carboxylesterase 1 (CES1) hydrolyzes the carboxylic acid substrate, 4-nitrophenyl acetate. The increase of hydrolyzed product is monitored by an increase in absorbance at 400 nm. |
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| Antigen References | Burgaleta C, et al. 1999. Cancer. 86:2133. Oertel J, et al. 1985. B J Haem. 61:717. Redinbo MR, et al. 2003. Biochem Soc Trans. 31:620. Satoh T, Hosokawa M, 2006. Chem Biol Interact. 162:195. Wang X, et al. 2017. Pharmacogenomics J. 16:2220 Orcholski ME, et al. 2017. Am J Physiol Lung Cell Mol Physiol. 313:L252. Wang X, et al. 2019. Proteomics. 19:e1800288. |
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| Gene ID | 1066 |
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| UniProt | View information about CES1 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Liver Carboxylesterase 1, Serine Esterase 1 (SES1), Human Monocyte Serine Esterase (HMSE), Cholesterol Ester Hydrolase (CESH) |
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