Biolegend, 796604, Biotinylated Recombinant Human CD155-Fc Chimera (carrier-free)
25microg
Catalog Number: 796604
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| Source | Human CD155, amino acid (Asp28-Asn343) (Accession: NP_006496.4), with a linker (GS), a C-terminal human IgG1 (Pro100-Lys330) and an Avi-tag, was expressed in CHO cells. Human CD155-Avi tag was site-specifically biotinylated by enzyme BirA. |
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| Molecular Mass | The 564 amino acid recombinant protein has a predicted molecular mass of approximately 62.3 kD. The DTT-reduced and non-reduced protein migrates at approximately 90 kD and 180 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Asp. |
| Purity | > 95%, as determined by Coomassie stained SDS-PAGE |
| Formulation | 0.22 µm filtered protein solution in PBS, pH 7.2, 10% glycerol. |
| Endotoxin Level | Less than 0.1 EU per µg of protein as determined by the LAL method |
| Concentration | 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Storage & Handling |
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| Unopened vial can be stored between 2°C and 8°C for up to 2 weeks at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Activity | Biotinylated recombinant human CD155-Fc chimera binds to immobilized recombinant human TIGIT at 2 µg/mL in a dose-dependent manner. The ED50 for this effect is 20 – 100 ng/mL. |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Homodimer |
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| Distribution | Endothelial cells, cancer cells, widely distributed |
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| Function | Regulator of the cell-mediated immune response, takes part in the transendothelial migration of leukocytes, mediates NK cell adhesion and activation. Acts as receptor of poliovirus. |
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| Interaction | T cells, NK, cytotoxic T lymphocytes (CTL) |
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| Ligand/Receptor | TIGIT, CD96, CD226 |
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| Bioactivity | Biotinylated recombinant human CD155-Fc chimera binds to immobilized recombinant human TIGIT in a dose-dependent manner using a functional ELISA. |
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| Cell Type | Dendritic cells, Th17, Thymocytes |
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| Biology Area | Adaptive Immunity, Cell Adhesion, Cell Motility/Cytoskeleton/Structure, Neuroscience |
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| Molecular Family | Adhesion Molecules, Soluble Receptors |
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| Antigen References | Meldenson CL, et al. 1989. Cell 56:855. Bottino C, et al. 2003. J Exp Med. 2003 198:557. Oda T, et al. 2004. Biochem Biophys Res Commun. 319:1253. Tahara-Hanaoka S, et al. 2006. Blood. 107:1491. Sullivan DP, et al. 2013. Am J Pathol. 182:1031. A Iguchi-Manaka, et al. 2016. PloS One 11(4): e0152982. Bowers JR, et al. 2017. Virus Res. 242:1. |
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| Gene ID | 5817 |
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| UniProt | View information about CD155 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Poliovirus Receptor, PVS, Nectin-Like 5, NECL-5 |
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