Biolegend, 800704, Biotin anti-β-Amyloid, 17-24 Antibody (Previously Covance catalog# SIG-39240)
200microl
Catalog Number: 800704
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| Verified Reactivity | Human, Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution (no preservatives or carrier proteins). |
| Preparation | The antibody was purified by affinity chromatography. |
| Concentration | 1 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody. |
| Application | IHC-P - Quality tested Direct ELISA - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 2.0 µg/ml is suggested. For Direct ELISA applications, a concentration range of 0.5 - 2.0 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Application Notes |
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| This antibody is reactive to amino acid residues 17-24 of ß amyloid. The epitope lies within amino acids 18-22 of ß amyloid (VFFAE). 4G8 ß-amyloid antibody reacts to abnormally processed isoforms, as well as precursor forms. This antibody clone has been reported for use on IHC of free-floating sections in PBS containing 1% Triton incubated with 0.1 m citrate buffer (4). Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissue sections (IHC-F) and immunocytochemistry (ICC) |
| Additional Product Notes | View more applications data for this product in our Scientific Poster Library. |
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| Application References(PubMed link indicates BioLegend citation) | Poduslo JF, et al. 2004. Biochem. 43:6064. (IHC-F) PubMed Forny-Germano L, et al. 2014. J Neurosci. 34:13629. (IHC-Other) PubMed Vallino Costassa E, et al. 2016. J Alzheimers Dis. 51: 875-87. (IHC-P) PubMed Chen X, et al. 2013. Neurobiol Aging. 34:2370. (ICC) PubMed Hatami A, et al. 2016. J Alzheimers Dis. 50:517. (IHC-P) PubMed Kawarabayashi T, et al. 2001. J Neurosci 21:372 (IP) Fonte V, et al. 2002. PNAS. 110:4853 (IP) |
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| Product Citations | Sun XY, et al. 2023. Signal Transduct Target Ther. 8:30. PubMed Lam S, et al. 2022. Acta Neuropathol Commun. 10:112. PubMed Townsend M, et al. 2010. J Pharmacol Exp Ther. 333:110:119. PubMed Jung ES, et al. 2022. Aging Cell. 21:e13623. PubMed Chen J, et al. 2019. Int J Mol Med. 44:313. PubMed Elmer BM, et al. 2019. PLoS One. 14:e0226245. PubMed Yao Q, et al. 2018. Acta Biochim Biophys Sin (Shanghai). 50(5):447-455. PubMed Watanabe H, et al. 2012. J Neurosci. 32:5085-5096. PubMed Hong J, et al. 2016. J Lipid Res. 57: 36 - 45. PubMed Clayton K, et al. 2021. Mol Neurodegener. 18:16. PubMed Bales K, et al. 2016. Brain. 139: 563 - 577. PubMed Luo M, et al. 2022. Genes Dis. 9:1315. PubMed Forgrave LM, et al. 2022. Clin Chem Lab Med. 60:198. PubMed Do TM, et al. 2020. Mol Ther Methods Clin Dev. 19:58. PubMed |
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| RRID | AB_2564650 (BioLegend Cat. No. 800704) AB_2564651 (BioLegend Cat. No. 800705) AB_2564649 (BioLegend Cat. No. 800706) |
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| Structure | Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD. |
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| Distribution | Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, and muscle. Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus. |
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| Function | The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent. |
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| Biology Area | Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation |
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| Molecular Family | APP/β-Amyloid |
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| Antigen References | Kumar A, et al. 2015. Pharmacol Rep. 67(2):195. Sadigh-Eteghad S, et al. 2015. Med Princ Pract. 24(1):1 Hampel H, et al. 2015. Expert Rev Neruother. 15(1):83. Puig KL, et al. 2012. Exp Gerontol. 48(7): 608. Selkoe DJ, et al. 2016. EMBO Mol Med. 8(6):595. Walsh DM, et al. 2007. J Neurochem. 101(5):1172. |
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| Gene ID | 351 |
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| UniProt | View information about beta-Amyloid 17-24 on UniProt.org |
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| Clone | 4G8 |
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| Regulatory Status | RUO |
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| Workshop | HCDM listed |
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| Other Names | AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein |
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| Previously | Signet Catalog# 9240-02Signet Catalog# 9240-05Signet Catalog# 9240-10Covance Catalog# SIG-39240 |
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| Isotype | Mouse IgG2b, κ |
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