Biolegend, 800711, Anti-β-Amyloid, 17-24 Antibody (Previously Covance catalog# SIG-39200)
1ml
Catalog Number: 800711
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| Verified Reactivity | Human, Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Preparation | Ascites |
| Concentration | The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml. |
| Storage & Handling | Store at -20°C or below. Upon initial thawing, apportion into working aliquots and store at -20°C or below. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Do not store in frost-free freezers. |
| Application | IHC-P - Quality tested Direct ELISA - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:1000 - 1:5000 is suggested. For Direct ELISA applications, a dilution range of 1:100 - 1:1000 is recommended. It is recommended that the reagent be titrated for optimal performance for each application. |
| Application Notes |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
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| This antibody is reactive to amino acid residues 17-24 of ß amyloid. The epitope lies within amino acids 18-22 of ß amyloid (VFFAE). 4G8 ß-amyloid antibody reacts to abnormally processed isoforms, as well as precursor forms. This antibody clone has been reported for use on IHC of free-floating sections in PBS containing 1% Triton incubated with 0.1 m citrate buffer (4). |
| Application References(PubMed link indicates BioLegend citation) | Poduslo JF, et al. 2004. Biochem. 43:6064. (IHC-F) PubMed Forny-Germano L, et al. 2014. J Neurosci. 34:13629. (IHC-Other) PubMed Vallino Costassa E, et al. 2016. J Alzheimers Dis. 51: 875:87. (IHC-P) PubMed Chen X, et al. 2013. Neurobiol Aging. 34:2370. (ICC) PubMed Hatami A, et al. 2016. J Alzheimers Dis. 50:517. (IHC-P) PubMed |
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| Product Citations | Bluhm A, et al. 2022. Biomolecules. 12:. PubMed Tournier BB, et al. 2023. Aging Brain. 2:100045. PubMed Sato W, et al. 2023. Commun Biol. 6:383. PubMed Klakotskaia D, et al. 2018. PLoS One. 8:8755. PubMed Choi S, et al. 2015. PLoS One. 10: 0143854. PubMed Woelfle S, et al. 2021. Front Cell Neurosci. 15:789903. PubMed Taddei RN, et al. 2022. Acta Neuropathol Commun. 10:72. PubMed Koronyo-Hamaoui M, et al. 2020. Brain. 143:336. PubMed Martin L, et al. 2021. Cell Reports. 35(6):109121. PubMed Gkanatsiou E, et al. 2021. J Neurochem. 159:258. PubMed Jiwaji Z, et al. 2022. Nat Commun. 13:135. PubMed Morales R, et al. 2021. Mol Psychiatry. 26:5690. PubMed Whitehouse I, et al. 2016. PLoS One. 11: 0159119. PubMed Chaudry O, et al. 2022. Sci Rep. 12:19129. PubMed Robertson AS, et al. 2017. Neuromuscul Disord. 27:635. PubMed Mu L, et al. 2022. Int J Mol Sci. 23:. PubMed Abramowski D, et al. 2012. J Neurosci. 32:1273-1283. PubMed Gamez N, et al. 2022. Cells. 11:. PubMed Schaefer P, et al. 2016. PLoS One. 11:e0168157. PubMed Lee CYD et al. 2018. Neuron. 97(5):1032-1048 . PubMed Lessard C, et al. 2015. PLoS One. 10: 0144758. PubMed Boeddrich A et al. 2018. Cell chemical biology. 26(1):109-120 . PubMed Zhang J, et al. 2021. iScience. 24:103327. PubMed Tang Z, et al. 2019. Nat Commun. 10:2173. PubMed Scholtzova H, et al. 2017. J Neurosci. 37(4):936-959. PubMed Maxwell AM, et al. 2021. Acta Neuropathol Commun. 9:201. PubMed |
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| RRID | AB_2734547 (BioLegend Cat. No. 800708) AB_2565325 (BioLegend Cat. No. 800709) AB_2565326 (BioLegend Cat. No. 800710) AB_2565324 (BioLegend Cat. No. 800711) |
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| Structure | Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD. |
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| Distribution | Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, and muscle. Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus. |
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| Function | The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent. |
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| Biology Area | Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation |
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| Molecular Family | APP/β-Amyloid |
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| Antigen References | Kumar A, et al. 2015. Pharmacol Rep. 67(2):195. Sadigh-Eteghad S, et al. 2015. Med Princ Pract. 24(1):1 Hampel H, et al. 2015. Expert Rev Neruother. 15(1):83. Puig KL, et al. 2012. Exp Gerontol. 48(7): 608. Selkoe DJ, et al. 2016. EMBO Mol Med. 8(6):595. Walsh DM, et al. 2007. J Neurochem. 101(5):1172. |
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| Gene ID | 351 |
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| UniProt | View information about beta-Amyloid 17-24 on UniProt.org |
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| Clone | 4G8 |
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| Regulatory Status | RUO |
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| Workshop | HCDM listed |
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| Other Names | AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein |
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| Previously | Signet Catalog# 9200-02Signet Catalog# 9200-05Signet Catalog# 9200-10Covance Catalog# SIG-39200 |
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| Isotype | Mouse IgG2b, κ |
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