Biolegend, 801203, Alexa Fluor® 488 anti-Tubulin β 3 (TUBB3) Antibody (Previously Covance catalog# A488-435L)
100microl
Catalog Number: 801203
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Category
Pack Size435 L
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| Verified Reactivity | Human, Mouse, Rat |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | This antibody was raised against microtubules derived from rat brain. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Previous lots (B432255 and prior) of this product may have been formulated in PBS + 50% glycerol + 0.03% thimerosal. For further information please contact BioLegend Technical Support or Customer Service. |
| Concentration | 1.0 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested IHC-P, ICFC, 3D IHC - Verified SB - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a dilution of 1:500 is recommended. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: flow cytometry4, immunofluorescence microscopy1-5,7, immunohistochemistry5,7, Western blotting8, and spatial biology (IBEX)9,10. This antibody is well characterized and highly reactive to neuron specific Class III ß-tubulin (ßIII). TUJ1 does not identify ß-tubulin found in glial cells. TUJ1 recognizes an epitope located within the last 15 C-terminal residues8. |
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| Additional Product Notes | Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH). |
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| Application References(PubMed link indicates BioLegend citation) | Nishimura K, et al. 2017. PLoS One. 12(1): e0170568. (ICC) Jongbloets J, et al. 2017. Nat Commun. 8: 14666. (ICC) PubMed Liu W.J, et al. 2015. Eur J Histochem. 59(1): 2464. (ICC) PubMed Chintalapudi SR, et al. 2016. Front Aging Neursci. 8:93. (FC, ICC) PubMed Ambasudhan R, et al. 2011. Cell Stem Cell. 9(2):113. (IHC, ICC) Hu X., et al. 2006. Nature Neuroscicene. 9(12):1520. (WB) PubMed Zechner D., et al. 2003. Develop Biology. 258(2):406. (ICC, IHC) Lee MK, et al. 1990. Proc. Natl. Acad. Sci. USA 18:7195. (WB) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Roque CG, et al. 2023. Sci Adv. 9:eadd2671. PubMed Vereertbrugghen A, et al. 2023. J Neuroinflammation. 20:120. PubMed Ruan H, et al. 2023. Acta Pharm Sin B. 13:2202. PubMed Saraswathula A, et al. 2023. Int Forum Allergy Rhinol. 13:230. PubMed Tokutake K, et al. 2022. Int J Mol Sci. 23: . PubMed Lu R, et al. 2021. Int J Mol Sci. 22:00. PubMed Giacomini C, et al. 2016. Mol Biol Cell. 27: 35 - 47. PubMed McCurdy EP et al. 2019. Cell Rep. 29(2):363-377 . PubMed Babetto E, et al. 2020. Nat Neurosci. 23:1215. PubMed Rocktäschel P, et al. 2019. Epilepsy Behav. 101:106581. PubMed Guzmán M, et al. 2020. Immunology. 161:148. PubMed Kim Y, et al. 2016. Sci Rep. 6: 23799. PubMed Inlender T, et al. 2021. Scientific Reports. 11(1):12796. PubMed Arsić A, et al. 2020. Sci Rep. 10:6441. PubMed Wang S, et al. 2022. PLoS Pathog. 18:e1010281. PubMed Stachtea X, et al. 2015. PLoS One. 10: e0140279. PubMed Larhammar M et al. 2017. eLife. 6 pii: e20725. PubMed Jalilian E, et al. 2022. Stem Cell Res Ther. 13:425. PubMed Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed Martínez JC et al. 2019. Neuron. 104(5):931-946 . PubMed Geisler S, et al. 2019. JCI Insight. 4:e129920. PubMed Žiak J, et al. 2020. EMBO Rep. e48512:21. PubMed Chung KM, et al. 2022. Cell Rep. 41:111488. PubMed |
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| RRID | AB_2564757 (BioLegend Cat. No. 801203) |
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| Disclaimer | Covered by US patents 5,675,063 and 7,429,487. Sold under license from Epitomics. |
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| Structure | Tubulin β 3 is a 450 amino acid protein with a molecular mass of ~50 kD. |
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| Distribution | Tissue distribution: central and peripheral nervous system. Cellular distribution: cytosol, cytoskeleton and nucleus. |
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| Function | Tubulin β 3 is the major constituent of microtubules, and plays a critical role in proper axon guidance and maintenance. |
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| Interaction | Alpha tubulin, kinesin and dynein. |
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| Cell Type | Mature Neurons, Neural Stem Cells |
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| Biology Area | Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells |
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| Molecular Family | Microtubules |
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| Antigen References | 1. Zhao X, et al. 2017. Med Sci Monit. 22: 3915. 2. Lebok P, et al. 2016. Oncol Lett. 11(3):1987. 3. Du J, et al. 2015. BMC Cancer. 15:536. PubMed 4. Rogue DM., et al. 2013. Clin Exp Metastasis. 31(1): 101. 5. Ploussard G, et al. 2010. Cancer Res. 70(22):9253. PubMed |
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| Gene ID | 10381 |
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| UniProt | View information about Tubulin beta-3 on UniProt.org |
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| Clone | TUJ1 |
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| Regulatory Status | RUO |
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| Other Names | CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, Tubulin beta-3 chain, tubulin beta-III, tubulin beta-4 chain, class III beta-tubulin |
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| Previously | Covance Catalog# A488-435L |
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| Isotype | Mouse IgG2a, κ |
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