Biolegend, 801207, Alexa Fluor® 594 anti-Tubulin β 3 (TUBB3) Antibody
25microg
Catalog Number: 801207
Send an RFQ.
Category
Loading…
| Verified Reactivity | Human, Mouse, Rat |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | This antibody was raised against microtubules derived from rat brain. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing no preservatives solution. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested 3D IHC, IHC-P - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 1.0 μg/ml is recommended. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Application Notes | Additional reported applications (for the relevant formats) include: flow cytometry4, immunofluorescence microscopy1-5,7, immunohistochemistry5,7, Western blotting8, and spatial biology (IBEX)9,10. This antibody is well characterized and highly reactive to neuron specific Class III ß-tubulin (ßIII). TUJ1 does not identify ß-tubulin found in glial cells. TUJ1 recognizes an epitope located within the last 15 C-terminal residues8. |
|---|
| Application References(PubMed link indicates BioLegend citation) | Nishimura K, et al. 2017. PLoS One. 12(1): e0170568. (ICC) Jongbloets J, et al. 2017. Nat Commun. 8: 14666. (ICC) PubMed Liu W.J, et al. 2015. Eur J Histochem. 59(1): 2464. (ICC) PubMed Chintalapudi SR, et al. 2016. Front Aging Neursci. 8:93. (FC, ICC) PubMed Ambasudhan R, et al. 2011. Cell Stem Cell. 9(2):113. (IHC, ICC) Hu X., et al. 2006. Nature Neuroscicene. 9(12):1520. (WB) PubMed Zechner D., et al. 2003. Develop Biology. 258(2):406. (ICC, IHC) Lee MK, et al. 1990. Proc. Natl. Acad. Sci. USA 18:7195. (WB) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
|---|
| Product Citations | Mubariz F, et al. 2023. Front Neurosci. 17:1152503. PubMed Santos TE, et al. 2020. Cell Reports. 32(3):107907. PubMed LaClair KD, et al. 2020. Acta Neuropathol. 140:121. PubMed |
|---|
| RRID | AB_2650635 (BioLegend Cat. No. 801207) AB_2650636 (BioLegend Cat. No. 801208) |
|---|
| Disclaimer | Covered by US patents 5,675,063 and 7,429,487. Sold under license from Epitomics. |
|---|
| Structure | Tubulin β 3 is a 450 amino acid protein with a molecular mass of ~50 kD. |
|---|
| Distribution | Tissue distribution: central and peripheral nervous system. Cellular distribution: cytosol, cytoskeleton and nucleus. |
|---|
| Function | Tubulin β 3 is the major constituent of microtubules, and plays a critical role in proper axon guidance and maintenance. |
|---|
| Interaction | Alpha tubulin, kinesin and dynein. |
|---|
| Cell Type | Mature Neurons, Neural Stem Cells |
|---|
| Biology Area | Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells |
|---|
| Molecular Family | Microtubules |
|---|
| Antigen References | 1. Zhao X, et al. 2017. Med Sci Monit. 22: 3915. 2. Lebok P, et al. 2016. Oncol Lett. 11(3):1987. 3. Du J, et al. 2015. BMC Cancer. 15:536. PubMed 4. Rogue DM., et al. 2013. Clin Exp Metastasis. 31(1): 101. 5. Ploussard G, et al. 2010. Cancer Res. 70(22):9253. PubMed |
|---|
| Gene ID | 10381 |
|---|
| UniProt | View information about Tubulin beta-3 on UniProt.org |
|---|
| Clone | TUJ1 |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, Tubulin beta-3 chain, tubulin beta-III, tubulin beta-4 chain, class III beta-tubulin |
|---|
| Isotype | Mouse IgG2a, κ |
|---|
