Biolegend, 801208, Alexa Fluor® 594 anti-Tubulin β 3 (TUBB3) Antibody
100microg
Catalog Number: 801208
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| Verified Reactivity | Human, Mouse, Rat |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | This antibody was raised against microtubules derived from rat brain. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing no preservatives solution. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested 3D IHC, IHC-P - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 1.0 μg/ml is recommended. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Application Notes | Additional reported applications (for the relevant formats) include: flow cytometry4, immunofluorescence microscopy1-5,7, immunohistochemistry5,7, Western blotting8, and spatial biology (IBEX)9,10. This antibody is well characterized and highly reactive to neuron specific Class III ß-tubulin (ßIII). TUJ1 does not identify ß-tubulin found in glial cells. TUJ1 recognizes an epitope located within the last 15 C-terminal residues8. |
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| Application References(PubMed link indicates BioLegend citation) | Nishimura K, et al. 2017. PLoS One. 12(1): e0170568. (ICC) Jongbloets J, et al. 2017. Nat Commun. 8: 14666. (ICC) PubMed Liu W.J, et al. 2015. Eur J Histochem. 59(1): 2464. (ICC) PubMed Chintalapudi SR, et al. 2016. Front Aging Neursci. 8:93. (FC, ICC) PubMed Ambasudhan R, et al. 2011. Cell Stem Cell. 9(2):113. (IHC, ICC) Hu X., et al. 2006. Nature Neuroscicene. 9(12):1520. (WB) PubMed Zechner D., et al. 2003. Develop Biology. 258(2):406. (ICC, IHC) Lee MK, et al. 1990. Proc. Natl. Acad. Sci. USA 18:7195. (WB) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Mubariz F, et al. 2023. Front Neurosci. 17:1152503. PubMed Santos TE, et al. 2020. Cell Reports. 32(3):107907. PubMed LaClair KD, et al. 2020. Acta Neuropathol. 140:121. PubMed |
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| RRID | AB_2650635 (BioLegend Cat. No. 801207) AB_2650636 (BioLegend Cat. No. 801208) |
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| Disclaimer | Covered by US patents 5,675,063 and 7,429,487. Sold under license from Epitomics. |
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| Structure | Tubulin β 3 is a 450 amino acid protein with a molecular mass of ~50 kD. |
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| Distribution | Tissue distribution: central and peripheral nervous system. Cellular distribution: cytosol, cytoskeleton and nucleus. |
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| Function | Tubulin β 3 is the major constituent of microtubules, and plays a critical role in proper axon guidance and maintenance. |
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| Interaction | Alpha tubulin, kinesin and dynein. |
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| Cell Type | Mature Neurons, Neural Stem Cells |
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| Biology Area | Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells |
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| Molecular Family | Microtubules |
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| Antigen References | 1. Zhao X, et al. 2017. Med Sci Monit. 22: 3915. 2. Lebok P, et al. 2016. Oncol Lett. 11(3):1987. 3. Du J, et al. 2015. BMC Cancer. 15:536. PubMed 4. Rogue DM., et al. 2013. Clin Exp Metastasis. 31(1): 101. 5. Ploussard G, et al. 2010. Cancer Res. 70(22):9253. PubMed |
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| Gene ID | 10381 |
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| UniProt | View information about Tubulin beta-3 on UniProt.org |
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| Clone | TUJ1 |
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| Regulatory Status | RUO |
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| Other Names | CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, Tubulin beta-3 chain, tubulin beta-III, tubulin beta-4 chain, class III beta-tubulin |
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| Isotype | Mouse IgG2a, κ |
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