Biolegend, 803013, Alexa Fluor® 488 anti-β-Amyloid, 1-16 Antibody (Previously Covance catalog# SIG-39347)
200microl
Catalog Number: 803013
Send an RFQ.
Category
Loading…
| Verified Reactivity | Human |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Concentration | 1 mg/mL |
| Storage & Handling | Store between 2-8°C. |
| Application | IHC-P - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
| Application Notes | This antibody is reactive to amino acid residue 1-16 of beta amyloid. The epitope lies within amino acids 3-8 of beta amyloid (EFRHDS). This antibody clone has been reported for use in immunohistochemistry of free-floating sections2,13. |
| Additional Product Notes | View more applications data for this product in our Scientific Poster Library. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Application References(PubMed link indicates BioLegend citation) |
|---|
| Thakker DR, et al. 2009. Proc. Natl. Acad. Sci. USA. 106(11):4501-6. (IHC) PubMed Oddo S, et al. 2005. Proc. Natl. Acad. Sci. USA. 102(8):3046-51. (IHC-other) PubMed Herzig M, et al. 2004. Nat. Neuro. 7(9):954-959. (WB) PubMed Zheng Y, et al. 2012. PLoS One 6:39035. (IHC-F) PubMed Abramowksi D, et al. J Neurosci. 32:1273. (WB) PubMed Forny-Germano L, et al. 2014. J. Neurosci. 34:13629. (WB, IHC) PubMed Gowert NS, et al. 2014. PLoS One 2:e90523. (ICC, EM) PubMed Sandoval-Hernández A, et al. 2015. PLoS One. 10: 0145467. (IHC-F) Kumar R, et al. 2016. Brain. 139:174-92 (WB) Miyamoto T, et al. 2016. J. Biol. Chem. 291:1719-34. (WB) Saito S, et al. 2017. Acta Neuropathol. Commun. 5:26-9. (IHC-P) PubMed Omata Y, et al. 2016. Aging (Albany NY) 8(3):427. (IHC-P) PubMed Peng W, et al. 2016. Neurobiol. Dis. 93:215. (IHC-other) PubMed Mandler M, et al. 2015. PLoS One. e0115237. (WB, IHC, ELISA) PubMed |
| Product Citations | Ohno Y, et al. 2022. J Vis Exp. . PubMed Lee JH, et al. 2022. EBioMedicine. 77:103903. PubMed Frame G, et al. 2022. J Alzheimers Dis. 88:471. PubMed Cramer PE et al. 2017. Neurobiology of aging. 64:92-106 . PubMed Paolicelli RC et al. 2017. Neuron. 95(2):297-308 . PubMed Fujita K, et al. 2016. Sci Rep. 6:31895. PubMed Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed McAlpine CS, et al. 2021. Nature. 595:701. PubMed Paulus A, et al. 2021. Int J Mol Sci. 22:. PubMed Abe Y, et al. 2020. Acta Neuropathol Commun. 8:67. PubMed Chen WT, et al. 2020. Cell. 182(4):976-991.e19. PubMed |
|---|
| RRID | AB_2564765 (BioLegend Cat. No. 803013) |
|---|
| Structure | Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD. |
|---|
| Distribution | Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, muscle. Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus. |
|---|
| Function | The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent. |
|---|
| Biology Area | Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation |
|---|
| Molecular Family | APP/β-Amyloid |
|---|
| Antigen References | Kumar A, et al. 2015. Pharmacol. Rep. 67(2):195. Sadigh-Eteghad S, et al. 2015. Med. Princ. Pract. 24(1):1 Hampel H, et al. 2015. Expert Rev. Neurother. 15(1):83. Puig KL, et al. 2012. Exp. Gerontol. 48(7): 608. Selkoe DJ, et al. 2016. EMBO Mol. Med. 8(6):595. Walsh DM, et al. 2007. J. Neurochem. 101(5):1172. |
|---|
| Gene ID | 351 |
|---|
| UniProt | View information about beta-Amyloid 1-16 on UniProt.org |
|---|
| Clone | 6E10 |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein |
|---|
| Previously | Covance Catalog# SIG-39347Signet Catalog# 9347-02 |
|---|
| Isotype | Mouse IgG1, κ |
|---|
