Biolegend, 803015, Anti-β-Amyloid, 1-16 Antibody (Previously Covance catalog# SIG-39300)
200microl
Catalog Number: 803015
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Category
| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Preparation | Ascites |
| Concentration | The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml. |
| Storage & Handling | Store at -20°C or below. Upon initial thawing, apportion into working aliquots and store at -20°C or below. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Do not store in frost-free freezers. |
| Application | WB - Quality tested Direct ELISA, IHC-P - Verified IHC-F, EM - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution of this reagent is 1:1,000 to 1:20,000. For Direct ELISA, the suggested dilution of this reagent is 1:10,000 to 1:100,000. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, the suggested dilution of this reagent is 1:1,000 to 1:20,000. It is recommended that the reagent be titrated for optimal performance for each application. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Application Notes |
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| This antibody is reactive to amino acid residue 1-16 of beta amyloid. The epitope lies within amino acids 3-8 of beta amyloid (EFRHDS). This antibody clone has been reported for use in immunohistochemistry of free-floating sections2,13. |
| Application References(PubMed link indicates BioLegend citation) | Thakker DR, et al. 2009. Proc. Natl. Acad. Sci. USA. 106(11):4501-6. (IHC) PubMed Oddo S, et al. 2005. Proc. Natl. Acad. Sci. USA. 102(8):3046-51. (IHC-other) PubMed Herzig M, et al. 2004. Nat. Neuro. 7(9):954-959. (WB) PubMed Zheng Y, et al. 2012. PLoS One 6:39035. (IHC-F) PubMed Abramowksi D, et al. J Neurosci. 32:1273. (WB) PubMed Forny-Germano L, et al. 2014. J. Neurosci. 34:13629. (WB, IHC) PubMed Gowert NS, et al. 2014. PLoS One 2:e90523. (ICC, EM) PubMed Sandoval-Hernández A, et al. 2015. PLoS One. 10: 0145467. (IHC-F) Kumar R, et al. 2016. Brain. 139:174-92 (WB) Miyamoto T, et al. 2016. J. Biol. Chem. 291:1719-34. (WB) Saito S, et al. 2017. Acta Neuropathol. Commun. 5:26-9. (IHC-P) PubMed Omata Y, et al. 2016. Aging (Albany NY) 8(3):427. (IHC-P) PubMed Peng W, et al. 2016. Neurobiol. Dis. 93:215. (IHC-other) PubMed Mandler M, et al. 2015. PLoS One. e0115237. (WB, IHC, ELISA) PubMed |
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| Product Citations | Fu W, et al. 2018. Exp Ther Med. 128:2252. PubMed Lana E, et al. 2019. Front Mol Neurosci. 12:176. PubMed Ren Z, et al. 2020. Mol Med Rep. 22:201. PubMed Tanaka H, et al. 2020. Nat Commun. 11:507. PubMed Bishay J, et al. 2022. Sci Rep. 12:15287. PubMed Li S, et al. 2022. Neural Plast. 2022:3995227. PubMed Jin J, et al. 2023. Gut Microbes. 15:2167172. PubMed Yao SY, et al. 2023. Br J Pharmacol. 180:1878. PubMed Na D, et al. 2023. Front Neurosci. 17:1106570. PubMed Ren X, et al. 2022. J Neuroinflammation. 19:61. PubMed Tang T, et al. 2022. J Virol. 96:e0168821. PubMed Huang S, et al. 2022. FEBS Lett. 596:1059. PubMed Januário YC, et al. 2022. J Biol Chem. 298:102172. PubMed Cai C, et al. 2022. J Neurosci. 42:6090. PubMed Lucero EM, et al. 2022. iScience. 25:105288. PubMed Hu Y, et al. 2022. Nat Metab. 4:1756. PubMed Reddy TT, et al. 2023. Molecules. 28: . PubMed Bathini P, et al. 2023. Brain Behav Immun Health. 28:100568. PubMed Lin W, et al. 2023. J Neuroinflammation. 20:21. PubMed Chen SH, et al. 2022. Neurosci Bull. 38:290. PubMed Arber C, et al. 2019. Brain Commun. 1:fcz024. PubMed Lai RH, et al. 2021. Aging Cell. 20:e13509. PubMed Forny-Germano L, et al. 2014. J Neurosci. 34:13629-13643. PubMed Beraldo F, et al. 2016. J Biol Chem. 291: 21945 - 21955. PubMed NULL, et al. 2021. Mol Imaging Biol. 23:991. PubMed Cone AS, et al. 2021. Theranostics. 11:8129. PubMed Zhang H, et al. 2016. J Neurosci. 36: 11837 - 11850. PubMed Robert J et al. 2017. eLife. 6 pii: e29595. PubMed Mukherjee J, et al. 2021. Synapse. 75:e22183. PubMed Zheng Y, et al. 2012. PLoS One. 7:e39035. PubMed Soto-Mercado V, et al. 2020. J Alzheimers Dis. . PubMed Shen Y, et al. 2020. Theranostics. 10:11794. PubMed Na H, et al. 2021. Am J Alzheimers Dis Other Demen. 36:15333175211012867. PubMed Shen Q, et al. 2022. Aging Cell. 21:e13573. PubMed Fujita K, et al. 2016. Sci Rep. 6:31895. PubMed Gowert N, et al. 2014. PLoS One. 9:e90523. PubMed Suh J, et al. 2020. Cell. 178(5):1159-1175.e17.. PubMed Nguyen GAH, et al. 2022. Molecules. 27:. PubMed Shang Y, et al. 2022. Acta Neuropathol. 144:911. PubMed Lyra E Silva NM, et al. 2021. Transl Psychiatry. 251:11. PubMed Benthem SD, et al. 2020. Current Biology. 30(13):2588-2601.e5. PubMed Cheng WH, et al. 2019. Alzheimers Res Ther. 11:6. PubMed Soto-Mercado V, et al. 2021. Biomolecules. 11:. PubMed Abramowski D, et al. 2012. J Neurosci. 32:1273-1283. PubMed Zhang J, et al. 2021. Aging Cell. 20:e13380. PubMed Moelgg K, et al. 2021. Biomolecules. 11:. PubMed Zhang X, et al. 2020. Sci Adv. 6:00. PubMed Kaur H, et al. 2021. Bioorg Med Chem Lett. 46:128164. PubMed Casali BT, et al. 2020. Neurobiol Dis. 142:104956. PubMed Quartey MO, et al. 2021. Sci Rep. 0.757638889. PubMed Klohs J, et al. 2016. J Cereb Blood Flow Metab. 36: 1614 - 1624. PubMed Qiu Y, et al. 2022. Front Aging Neurosci. 14:896522. PubMed Kumar S, et al. 2022. NPJ Genom Med. 7:47. PubMed Chan E, et al. 2016. Sci Rep. 6: 26119. PubMed Lee CYD et al. 2018. Neuron. 97(5):1032-1048 . PubMed Ippati S, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed Boeddrich A et al. 2018. Cell chemical biology. 26(1):109-120 . PubMed Zhang Y, et al. 2022. Bioact Mater. 11:192. PubMed Seino S, et al. 2018. Biomed Res. 39:105. PubMed Lin H, et al. 2022. Front Aging Neurosci. 14:885145. PubMed Scholtzova H, et al. 2017. J Neurosci. 37(4):936-959. PubMed Heiss J, et al. 2016. Cereb Cortex. 10.1093/cercor/bhw188. PubMed Meilandt WJ, et al. 2020. J Neurosci. 40:1956. PubMed Casali BT, et al. 2018. J Neuroinflammation. 15:43. PubMed Arber C, et al. 2021. Cell Reports. 34(2):108615. PubMed Jang J, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.08.127. PubMed Xiao Zhang et al. 2017. The Journal of Neuroscience. 37(19):4928-4941 . PubMed Actor-Engel HS, et al. 2021. Eneuro. Online ahead of print. PubMed Willuweit A, et al. 2021. Front Neurosci. 15:699926. PubMed Dai Y, et al. 2020. Front Cell Dev Biol. 8:593659. PubMed Song JX, et al. 2020. Aging Cell. 19:e13069. PubMed Li N, et al. 2019. Neural Regen Res. 14:1037. PubMed Magalhães J, et al. 2021. Mol Psychiatry. Online ahead of print. PubMed Arber C, et al. 2020. Mol Psychiatry. 25:2919. PubMed Su Q, et al. 2021. Alzheimers Res Ther. 13:07. PubMed Porter KN, et al. 2020. Front Aging Neurosci. 12:92. PubMed Ye T, et al. 2021. Commun Biol. 4:195. PubMed Ge W, et al. 2021. Am J Transl Res. 13:1471. PubMed Li T, et al. 2016. Nat Commun. 7:12082. PubMed Soto-Mercado V, et al. 2020. PLoS One. 15:e0221669. PubMed Kordower JH, et al. 2017. Ann Neurol. 81:46. PubMed Williamson RL, et al. 2017. J Biol Chem. 292:19873. PubMed Lai RH, et al. 2022. Aging Cell. 21:e13670. PubMed Peng D, et al. 2022. Theranostics. 12:3862. PubMed |
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| RRID | AB_2728527 (BioLegend Cat. No. 803014) AB_2565328 (BioLegend Cat. No. 803015) AB_2565329 (BioLegend Cat. No. 803016) AB_2565327 (BioLegend Cat. No. 803017) |
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| Structure | Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD. |
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| Distribution | Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, muscle. Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus. |
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| Function | The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent. |
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| Biology Area | Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation |
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| Molecular Family | APP/β-Amyloid |
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| Antigen References | Kumar A, et al. 2015. Pharmacol. Rep. 67(2):195. Sadigh-Eteghad S, et al. 2015. Med. Princ. Pract. 24(1):1 Hampel H, et al. 2015. Expert Rev. Neurother. 15(1):83. Puig KL, et al. 2012. Exp. Gerontol. 48(7): 608. Selkoe DJ, et al. 2016. EMBO Mol. Med. 8(6):595. Walsh DM, et al. 2007. J. Neurochem. 101(5):1172. |
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| Gene ID | 351 |
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| UniProt | View information about beta-Amyloid 1-16 on UniProt.org |
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| Clone | 6E10 |
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| Regulatory Status | RUO |
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| Other Names | AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein |
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| Previously | Signet Catalog# 9300-02Signet Catalog# 9300-05Signet Catalog# 9300-10Covance Catalog# SIG-39300 |
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| Isotype | Mouse IgG1, κ |
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