Biolegend, 836309, Alexa Fluor® 594 anti-SNAP-25 Antibody
25microg
Catalog Number: 836309
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| Verified Reactivity | Human, Mouse, Rat |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Full length, unaltered SNAP-25 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | IHC-P - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm) |
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| Application References(PubMed link indicates BioLegend citation) | Postupna NO, et al. 2014. Lab Invest. 94:1161. (FC) Hirano AA, et al. 2011. J. Comp. Neurol. 519:972. (WB, IHC, EM) PubMed Flores-Otero J, et al. 2007. J. Neurosci. 27:14023. (IF) PubMed Rozas JL, et al. 2012. Neuron. 74:151. (IF) PubMed Brinkmalm A, et al. 2014. Mol. Neurodegener. 9:53. (IP) PubMed Gu Y, et al. 2016. Proc. Natl. Acad. Sci. U. S. A. E922-31. (WB) PubMed Kabachinski G, et al. 2016. Mol. Biol. Cell. 654-68.(IF) PubMed Kunii M, et al. 2016. J. Cell Biol. 215:121-138. (IP) PubMed |
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| RRID | AB_2750110 (BioLegend Cat. No. 836309) AB_2750111 (BioLegend Cat. No. 836310) |
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| Structure | SNAP-25 is a 206 amino acid protein with a molecular mass of 23 kD. |
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| Distribution | Tissue distribution: Mainly in the central nervous system, in neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum Cellular distribution: Cytosol, golgi apparatus, and cell membrane |
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| Function | SNAP-25 associates with proteins involved in vesicle docking and membrane fusion. It regulates plasma membrane recycling through its interaction with centromere protein F (CENPF). |
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| Interaction | In adult tissue, SMI 81 visualizes synapse-rich areas of brain and retina. In developing brain and cell cultures, SMI 81 reacts with neuronal cell bodies and axons. It is useful as an early marker for developing neurons in cell culture. |
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| Cell Type | Neurons |
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| Biology Area | Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology |
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| Molecular Family | Presynaptic proteins |
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| Antigen References | 1. Snyder DA, et al. 2006. Cell Biochem. Biophys. 45(1):111-23. 2. Nagy G, et al. 2004. Neuron. 41(3):417–29. |
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| Gene ID | 6616 |
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| UniProt | View information about SNAP-25 on UniProt.org |
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| Clone | SMI 81 |
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| Regulatory Status | RUO |
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| Other Names | Synaptosomal-associated protein 25, SUP, Synaptosomal-associated 25 kD Protein |
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| Isotype | Mouse IgG1, κ |
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