Biolegend, 836505, Alexa Fluor® 488 anti-Myelin Basic Protein Antibody
25microg
Catalog Number: 836505
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| Verified Reactivity | Human, Mouse, Rat |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | IHC-P - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes |
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| SMI 94 reacts with the Myelin Basic Protein (MBP) peptic fragment 70-89 of the classic human myelin basic protein sequence. SMI 94 detects developing and adult myelin, developing oligodendrocytes and distinguishes oligodendrocytes from astrocytes, microglia, neurons and other cells in brain sections. The combination of SMI 94 with SMI 91 and/or 99 is useful for immunocytochemical studies on the progression of normal and pathologic myelination. |
| Application References(PubMed link indicates BioLegend citation) | Kiryu-Seo S, et al. 2010. J. Neurosci. 30:6658. (ICC) Gensel JC, et al. 2009. J. Neurosci. 29:3956. (IHC-P) Wang H, et al. 2008. J. Cell. Biol. 182:1171. (ICC, WB) McLean NA, et al. 2014. PLoS One 9:e110174. (IHC-F) Tian C, et al. 2014. Front Cell Neurosci. 8:297. (IHC-P) Wüthrich C, et al. 2009. J. Neuropathol. Exp. Neurol. 68:15. (IHC, ICC) Maire CL, et al. 2014. Stem Cells 32:313. (IHC-P) Rusielewicz T, et. al. 2014. Glia 62:580. (ICC) Li C, et al. 2012. ASN Neuro. 4:e00102. (ICC) Huang JH, et al. 2009. Tissue Eng. Part A 15:1677. (IHC-P) Göttle P, et al. 2015. J. Neurosci. 35:906. (ICC) Zhao X, et al. 2010. Neuron. 65:612. (ICC) |
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| RRID | AB_2801174 (BioLegend Cat. No. 836505) AB_2801175 (BioLegend Cat. No. 836506) |
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| Structure | Myelin Basic Protein is a 304 amino acid protein with a molecular mass of 33kD. |
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| Distribution | Cytoskeleton, plasma membrane, cytosol, and extracellular. |
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| Function | Classic MBP isoforms (4-14) are the most abundant protein components of the myelin membrane in the CNS. They play a role in both its formation and stabilization. The non-classic isoforms may have a role in the early developing brain long before myelination. |
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| Biology Area | Cell Biology, Neuroscience, Neuroscience Cell Markers |
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| Gene ID | 4155 |
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| UniProt | View information about Myelin Basic Protein on UniProt.org |
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| Clone | SMI 94 |
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| Regulatory Status | RUO |
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| Other Names | Golli-MBP, myelin A1 protein, myelin membrane encephalitogenic protein |
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| Isotype | Mouse IgG1, κ |
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