Biolegend, 838101, Anti-CD171 (L1CAM) Antibody (Previously Covance catalog# MMS-172R)
500microl
Catalog Number: 838101
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| Verified Reactivity | Human, Mouse, Rat |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | This monoclonal antibody was raised against purified chick L1, and binds to the L1 cytoplasmic domain at about T1172 (adjacent to the YRSL sorting signal). |
| Preparation | Ascites |
| Concentration | The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml. |
| Storage & Handling | Store at -20°C. It is suggested that the total volume be divided into useable aliquots upon initial thaw. |
| Application | WB - Quality testedICC, IP, IHC-P - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution for this reagent is 1:2000. It is recommended that the reagent be titrated for optimal performance for each application. |
| Application Notes | Phosphorylation of either T1172 or Y1176 strongly inhibits binding of 74-5H7 to the L1 cytoplasmic domain. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
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| Application References(PubMed link indicates BioLegend citation) |
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| Kamiguchi H, et al. 1998. J. Neurosci. 18(14):5311-21. Lemmon V, et al. 1989. Neuron. 2(6):1597-603. Yip PM, Siu CH. 2001. J. Neurochem. 76(5):1552-64. Schaefer AW, et. al. 2002. J. Cell Biol. 157:1223. (WB, IHC-P, IP) Beer S, et. al. 1999. J. Cell Sci. 112:2667. (IP) Itoh K, et. al. 2005. Mol. Cell Neurosci. 29:245. (WB, IP) Zheng XJ, et. al. 2005. Environ. Technol. 26:1073. (ICC) Chen SY, et. al. 2001. FASEB. J. 15:1649. (IHC-P) PubMed Itoh K, et. al. 2004. J. Cell Biol. 165:145. (WB) Cohen NR, et. al. 1998. Curr. Biol. 8:26. (WB) |
| Product Citations | Chen S, et al. 2001. FASEB J. 15:1649-1561. PubMed Hu J, et al. 2022. Cell Death Dis. 13:48. PubMed Kleene R, et al. 2021. J Neurochem. 157:1102. PubMed Girbes Minguez M, et al. 2020. FASEB J. 34:9869. PubMed |
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| RRID | AB_2565388 (BioLegend Cat. No. 838101) |
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| Structure | Expected MW: 200-220 kD |
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| Biology Area | Cell Biology, Neuroscience, Synaptic Biology |
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| Molecular Family | Adhesion Molecules |
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| Gene ID | 3897 |
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| UniProt | View information about CD171 on UniProt.org |
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| Clone | 74-5H7 |
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| Regulatory Status | RUO |
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| Other Names | Neural cell adhesion molecule L1, antigen identified by monoclonal antibody R1 |
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| Previously | Covance Catalog# MMS-172R |
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| Isotype | Mouse IgG1, κ |
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