Biolegend, 847405, Alexa Fluor® 594 anti-ATG5 Antibody
25microg
Catalog Number: 847405
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| Verified Reactivity | Human, Mouse, Rat, Drosophila |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | ATG5 peptide conjugated to KLH. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Application Notes | Reactivity to Drosophila was only verified with the purified format. |
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| RRID | AB_2750295 (BioLegend Cat. No. 847405) AB_2750296 (BioLegend Cat. No. 847406) |
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| Structure | Human ATG5 is a 275 amino acid protein with a molecular mass of 32 kD. |
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| Distribution | Tissue distribution: Ubiquitous. Cellular distribution: Vacuole, lysosome, and to a lesser extent mitochondria. |
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| Function | ATG5 is involved in many cellular processes including autophagic vesicle formation, mitochondrial quality control after oxidative damage, innate anti-viral immune response, and apoptosis. |
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| Interaction | ATG12, LC3B, ATG16, TECPR1, Autophagy-linked FYVE protein. |
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| Biology Area | Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance |
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| Molecular Family | Autophagosome Markers |
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| Antigen References | 1. Uchiyama Y, et al. 2008. Histochem. Cell Biol. 129:407. PubMed 2. Wu H, et al. 2014. Int. J. Biol. Sci. 10:1072. PubMed 3. Subramani S, Malhotra V. 2013. EMBO Rep. 14(2):143. PubMed |
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| Gene ID | 9474 |
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| UniProt | View information about ATG5 on UniProt.org |
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| Clone | 177.19 |
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| Regulatory Status | RUO |
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| Other Names | Autophagy protein 5 |
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| Isotype | Mouse IgG1, κ |
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