Biolegend, 853309, Alexa Fluor® 594 anti-TMEM119 (Extracellular) Antibody
25microg
Catalog Number: 853309
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Recombinant human TMEM119 protein (extracellular domain) |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | IHC-P - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Application Notes |
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| For microscopy, the antibody was found to work best when using fresh frozen human tissues. Communication by Dr. Frederick Christian Bennett at Stanford University, under review in Neuron. *The human TMEM119 full length plasmid was a courtesy gift from Dr. Ben Barres at Stanford University. |
| Application References(PubMed link indicates BioLegend citation) | Bennett FC, et al. 2018. Neuron. 98:1-14 PubMed |
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| RRID | AB_3097500 (BioLegend Cat. No. 853309) AB_3097500 (BioLegend Cat. No. 853310) |
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| Structure | TMEM119 is an 283 amino acid protein with a molecular mass of 30 kD. |
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| Distribution | Tissue distribution: TMEM119 is expressed in the brain, bone, and lymphoid tissues. Cellular distribution: Membrane and endoplasmic reticulum. |
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| Function | TMEM119 is an important microglia marker and is implicated in neurodegenerative diseases. |
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| Interaction | TMEM119 interacts with SMAD1, SMAD5 and RUNX2. |
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| Biology Area | Cell Biology, Neuroscience, Neuroscience Cell Markers |
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| Antigen References | Bennett ML, et al. 2016. Proc Natl Acad Sci U S A. 113 Satoh J, et al. 2016. Neuropathology. 36:39-49 Kawao N and Kaji H. 2015. J Cell Biochem. 116:687 Zrzavy T, et al. 2017. Brain. 140:1900-1913 |
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| Gene ID | 338773 |
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| UniProt | View information about TMEM119 on UniProt.org |
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| Clone | A16075D |
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| Regulatory Status | RUO |
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| Other Names | Transmembrane protein 119 |
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| Isotype | Mouse IgG2b, κ |
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