Biolegend, 853317, Alexa Fluor® 488 anti-TMEM119 (Extracellular) Antibody
25microg
Catalog Number: 853317
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Recombinant human TMEM119 protein (extracellular domain) |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested ICC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | For microscopy, the antibody was found to work best when using fresh frozen human tissues. Communication by Dr. Frederick Christian Bennett at Stanford University, under review in Neuron. *The human TMEM119 full length plasmid was a courtesy gift from Dr. Ben Barres at Stanford University. |
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| Application References(PubMed link indicates BioLegend citation) | Bennett FC, et al. 2018. Neuron. 98:1-14 PubMed |
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| RRID | AB_3662340 (BioLegend Cat. No. 853317) AB_3662340 (BioLegend Cat. No. 853318) |
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| Structure | TMEM119 is an 283 amino acid protein with a molecular mass of 30 kD. |
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| Distribution | Tissue distribution: TMEM119 is expressed in the brain, bone, and lymphoid tissues. Cellular distribution: Membrane and endoplasmic reticulum. |
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| Function | TMEM119 is an important microglia marker and is implicated in neurodegenerative diseases. |
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| Interaction | TMEM119 interacts with SMAD1, SMAD5 and RUNX2. |
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| Biology Area | Cell Biology, Neuroscience, Neuroscience Cell Markers |
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| Antigen References | Bennett ML, et al. 2016. Proc Natl Acad Sci U S A. 113 Satoh J, et al. 2016. Neuropathology. 36:39-49 Kawao N and Kaji H. 2015. J Cell Biochem. 116:687 Zrzavy T, et al. 2017. Brain. 140:1900-1913 |
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| Gene ID | 338773 |
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| UniProt | View information about TMEM119 on UniProt.org |
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| Clone | A16075D |
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| Regulatory Status | RUO |
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| Other Names | Transmembrane protein 119 |
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| Isotype | Mouse IgG2b, κ |
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