Biolegend, 906116, Alexa Fluor® 647 anti-6-His Epitope Tag Antibody
100microg
Catalog Number: 906116
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Category
| Verified Reactivity | Epitope tag |
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| Reported Reactivity | Species independent |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Monoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.5 μg/mL (1:200-1:1,000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | The sequence of the protein flanking the poly His tag does not influence antibody binding. This antibody was subsequently purified via a protein-G column. |
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| Application References(PubMed link indicates BioLegend citation) | Deinert K, et al. 2001. J. Biol. Chem. 276:6000. (WB) Kojima H, et al. 2001. J. Biol. Chem.(22)276:19238. (ICC) PubMed Lapinski PE, et al. 2000. J. Biol. Chem. 275:6831. (WB) Bartsch D, et al. 1998. Cell. 95:211. (ICC) Fuentes EJ, et al. 2003. J Biol Chem. 278:2118. PubMed Montegna EA, et al. 2012 PLoS One.7:31156. (WB) PubMed Huang L, et al. 2013. Nat Biotechnol. 31:450. (WB) PubMed |
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| Product Citations | Wong JJ, et al. 2021. Sci Adv. 7:. PubMed |
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| RRID | AB_2750116 (BioLegend Cat. No. 906115) AB_2750117 (BioLegend Cat. No. 906116) |
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| Biology Area | Cell Biology |
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| Gene ID | NA |
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| UniProt | View information about 6-His on UniProt.org |
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| Clone | 6-His |
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| Regulatory Status | RUO |
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| Other Names | polyhistidine-tag, hexa histidine-tag, 6xHis-tag, His6 tag, His-tag(TM) |
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| Isotype | Mouse IgG1, κ |
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