Biolegend, 914207, TotalSeq™-Bn1301 anti-Pan-Cytokeratin Antibody
10microg
Catalog Number: 914207
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| Verified Reactivity | Human, Rat |
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| Reported Reactivity | Dog, Non-Human Primate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | This antibody was developed using human epidermal keratins. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA |
| Preparation | The antibody was purified by chromatography and conjugated with TotalSeq™-Bn oligomer under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze. |
| Application | SB - Quality tested |
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| Recommended Usage |
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| Each lot of this antibody is quality control tested by immunofluorescent staining in formalin-fixed, paraffin-embedded (FFPE) lymphoid tissue, and the oligomer sequence is confirmed by sequencing. TotalSeq™-Bn antibodies are compatible with the 10x Visium CytAssist Gene and Protein Expression Assay. To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution at 14,000xg at 2 − 8°C for 10 minutes before use. Carefully pipette out the liquid avoiding the bottom of the tube when handling. To determine and optimize dilutions for the addition of Totalseq™-Bn antibodies into pre-designed antibody panels, refer to 10x Genomics Custom Add-on Antibody Optimization guide. Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. |
| Additional Product Notes | TotalSeq™-Bn reagents are designed to profile protein levels following an optimized protocol in spatial transcriptomics. Compatible spatial biology devices (e.g. Imaging System, 10x Genomics Visium Spatial CytAssist Gene and Protein Expression instruments and reagents) and sequencer (e.g. Illumina analyzers) are required. TotalSeq™-B reagents are not compatible with the 10x Genomics Visium system. The complete barcode sequence may be provided upon request. Please contact technical support for more information, or visit TotalSeq™-Bn Reagents for 10x Genomics Visium CytAssist Gene and Protein Assay. |
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| RRID | AB_3083233 (BioLegend Cat. No. 914207) |
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| Biology Area | Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | Intermediate Filaments |
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| Gene ID | NA |
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| UniProt | View information about Pan-Cytokeratin on UniProt.org |
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| Clone | AE-1/AE-3 |
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| Regulatory Status | RUO |
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| Other Names | Pan-Cytokeratin (AE-1/AE-3), AE1/AE3 |
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| Isotype | Mouse IgG1, κ |
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| Barcode Sequence | AACGTAACAGCGATT |
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