Biolegend, 914213, Alexa Fluor® 488 anti-Pan-Cytokeratin Antibody
25microg
Catalog Number: 914213
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| Verified Reactivity | Human, Rat |
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| Reported Reactivity | Dog, Non-Human Primate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | This antibody was developed using human epidermal keratins. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | IHC-P - Quality tested ICC, ICFC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1-10 µg/mL is suggested. For immunocytochemistry, a concentration range of 1 - 10 μg/mL is recommended. For flow cytometric staining using our Cyto-Fast™ Fix/Perm Buffer Set, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Additional Product Notes | For use of this antibody (clone AE-1/AE-3) in immunocytochemistry (ICC), we recommend fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with either 0.5% Triton-X or 100% ice-cold methanol, or fixation/permeabilization with 100% ice-cold methanol alone. For use of this antibody (clone AE-1/AE-3) in intracellular flow cytometry (ICFC), we recommend fixation/permeabilization using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803). For use of this antibody (clone AE-1/AE-3) in immunohistochemistry (IHC), we recommend antigen retrieval with either Sodium Citrate H.I.E.R. (Cat. No. 928502) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422703). |
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| RRID | AB_3675198 (BioLegend Cat. No. 914213) AB_3675198 (BioLegend Cat. No. 914214) |
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| Distribution | Cytoplasm, cell membrane |
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| Function | Cytoskeleton, cell motility and structure |
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| Biology Area | Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | Intermediate Filaments |
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| Antigen References | Metcalf KJ, et al. 2022. Matrix Biol Plus. 14:100105. Jiang S, et al. 2022. Immunity. 55:1118. Tiago M, et al. 2020. Br J Cancer. 122:789. Kou R, et al. 2018. Oncol Rep. 2537-44 Xie S, et al. 2020. Ann Transl Med. 1.384027778. Chang TH, et al. 2020. Cell Death Dis. 11:338. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262-77. Cai S, et al. 2022. iScience. 25:104980. |
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| Gene ID | NA |
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| UniProt | View information about Pan-Cytokeratin on UniProt.org |
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| Clone | AE-1/AE-3 |
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| Regulatory Status | RUO |
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| Other Names | KRT, Pan-Cytokeratin (AE-1/AE-3), AE1/AE3, AE 1+AE 3, cytokeratin, P04264 |
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| Isotype | Mouse IgG1, κ |
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