Biolegend, 925903, AEC Substrate Buffer
50ml
Catalog Number: 925903
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| Storage & Handling | Store this product between 2 and 8°C. AEC preparations should be stored in a manner which minimizes exposure to light. |
|---|---|
| Application | IHC |
| Recommended Usage | Each lot of this product is quality control tested by immunohistochemistry. AEC Substrate Buffer is for use with AEC chromogen concentrate (Cat. No. 925804). The optimal working dilution should be determined for each specific assay condition. |
| Application Notes | AEC reaction product is soluble in alcohol. Do not use organic solvents after AEC staining. Protocol: 1. Dilute AEC concentrate (Cat. No. 925804) 1:50 with Substrate Buffer 2. Apply mixture to tissue section 3. Incubate the slides for 5-10 min. at room temperature 4. Rinse tissue sections with deionized water 5. Counterstain and mount using aqueous mounting media 6. AEC induces a red precipitate with almost no background |
| Product Citations | Costa B, et al. 2024. Nat Commun. 1745:15. PubMed |
| Gene ID | NA |
| Regulatory Status | RUO |
| Other Names | Substrate Buffer |
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