Biolegend, 927301, Avidin/Biotin Blocking System (Previously Covance catalog# SIG-31126)

Name: Avidin/Biotin Blocking System (Previously Covance catalog# SIG-31126)
Brand: Biolegend
SKU: 927301

VO15

Catalog Number: 927301

Pricing

Formulation	Blocking Kit. The Avidin and Biotin Solutions contain 0.09% sodium azide.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Storage & Handling	Store between 2°C and 8°C.
Application	IHC, WB
Recommended Usage	The optimal working dilution should be determined for each specific assay condition. Blocking Procedure: For paraffin-embedded tissues: To be performed after the antigen retrieval and serum block but before primary antibody incubation. 1. Incubate the Avidin Solution for 10-15 minutes at room temperature. 2. Incubate with the Biotin Solution for 10-15 minutes at room temperature.
Application Notes	BioLegend's Avidin/Biotin Blocking System is intended for use in immunohistochemistry and immunoblotting procedures. The purpose is to decrease the signal caused by endogenous avidin, biotin, or biotin-binding proteins when immunostaining specimens with detection systems that rely on avidin-biotin or steptavidin-biotin chemistry. Nonspecific staining due to these factors commonly resembles a diffuse, weak, cytoplasmic pattern.

Ordering

Unless otherwise stated, the minimum order amount is USD 1,000.
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