Biolegend, 934105, Alexa Fluor® 647 anti-HMOX1 Antibody
25tests
Catalog Number: 934105
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Formulation | µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC (test sizes) - Quality tested ICC (µg size) - Quality tested |
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| Recommended Usage |
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| Each lot of the test-sized antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Each lot of the µg-sized antibody is quality control tested by immunocytochemistry. For immunocytochemistry using the ug size, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Clone was tested for ICC using three fix/perm methods (PFA/Triton-X, PFA/MeOH, MeOH only), the best method was the use of PFA/Triton X-100. In the CoCl2 treated cells for ICC, staining was seen in less than 5% of cells. Clone was tested for ICFC using cytokine fix/perm method only (based on ICC results). |
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| RRID | AB_2941637 (BioLegend Cat. No. 934105) AB_2941637 (BioLegend Cat. No. 934106) AB_2941637 (BioLegend Cat. No. 934108) |
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| Distribution | Cytosol/Endoplasmic reticulum |
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| Biology Area | Cell Biology |
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| Molecular Family | Enzymes and Regulators |
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| Antigen References | 1. Yang B, et al. 2018. Toxicol Appl Pharmacol. 355:189-197 2. Chora A, et al. 2007. J Clin Invest. 117(2):438-47 3. Harding H, et al. 2003. Mol Cell. 11(3):619-33. |
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| Gene ID | 3162 |
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| UniProt | View information about HMOX1 on UniProt.org |
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| Clone | W19398C |
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| Regulatory Status | RUO |
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| Other Names | Heme Oxygenase 1, HO-1, Heat Shock Protein, 32-KD |
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| Isotype | Rat IgG2a, κ |
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