Biolegend, 939208, Alexa Fluor® 488 anti-NRF2 Antibody
100tests
Catalog Number: 939208
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Partial recombinant human NRF2 protein |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | This clone was tested for ICC on 4% PFA-fixed HeLa cells and permeabilized with either methanol or Triton X-100. Both permeabilization methods were compatible with NRF2 staining. Clone W19086B is validated to detect NRF2 in mouse NIH/3T3 cells using a Western blot on whole cell lysate. |
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| RRID | AB_3675199 (BioLegend Cat. No. 939207) AB_3675199 (BioLegend Cat. No. 939208) |
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| Structure | NRF2 is a 605 amino acid protein with a predicted molecular weight of 67 kD. |
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| Distribution | Ubiquitously expressed/Nucleus |
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| Function | Transcription factor/Redox balance |
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| Biology Area | Cell Biology, Mitochondrial Function, Transcription Factors |
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| Antigen References | M. da Costa R, et al. 2019. Front In Pharm. 10:3389. Shoemaker A. 2017. Sci Trans Med. 9:420. Robledinos-Antón N, et al. 2019. Hindawi. 10:1155. Cuadrado A, et al. 2019. Nat Rev Drug Discov. 18:295-317. |
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| Gene ID | 4780 |
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| UniProt | View information about NRF2 on UniProt.org |
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| Clone | W19086B |
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| Regulatory Status | RUO |
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| Other Names | Erythroid 2 Like 2, NF-E2-Related Factor 2, HEBP1, IMDDHH |
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| Isotype | Rat IgG2a, κ |
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