Biolegend, 940203, Alexa Fluor® 647 anti-ERG Antibody
25microg
Catalog Number: 940203
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Partial recombinant human ERG protein |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested ICFC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | For use of this antibody in Immunocytochemistry (ICC), we recommend fixation with PFA fixation Buffer (Cat. No. 420801) followed by permeabilization with either Triton X-100 (0.5 %) or ice-cold methanol. For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401) or True-Phos™ Perm Buffer (Cat. No. 425401). |
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| RRID | AB_3699101 (BioLegend Cat. No. 940203) |
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| Structure | ERG is a 449 amino acid protein with an expected molecular weight of 54 kD. |
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| Distribution | Ubiquitously expressed/ Nucleus |
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| Function | Transcription factor |
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| Cell Type | Endothelial cells |
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| Biology Area | Cell Biology, Ubiquitin/Protein Degradation |
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| Antigen References | 1. Adamo P, et al. 2016. J. Biol. Chem. 35:4013 2. Birdsey G, et al. 2015. Dev. Cell. 32:82 3. Shah A, et al. 2016. Vascul. Pharmacol. 86:3 |
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| Gene ID | 2078 |
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| UniProt | View information about ERG on UniProt.org |
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| Clone | W19256A |
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| Regulatory Status | RUO |
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| Other Names | ETS-Related Gene, Transcriptional Regulator ERG, ETS Transcription Factor ERG, Erythroblast Transformation-Specific Transcription Factor ERG Variant 10, p55 |
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| Isotype | Rat IgG2a, κ |
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