Biolegend, 940204, Alexa Fluor® 647 anti-ERG Antibody
100microg
Catalog Number: 940204
Send an RFQ.
Category
Loading…
| Verified Reactivity | Human |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Partial recombinant human ERG protein |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested ICFC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Excitation Laser | Red Laser (633 nm) |
|---|
| Application Notes | For use of this antibody in Immunocytochemistry (ICC), we recommend fixation with PFA fixation Buffer (Cat. No. 420801) followed by permeabilization with either Triton X-100 (0.5 %) or ice-cold methanol. For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401) or True-Phos™ Perm Buffer (Cat. No. 425401). |
|---|
| RRID | AB_3699101 (BioLegend Cat. No. 940203) |
|---|
| Structure | ERG is a 449 amino acid protein with an expected molecular weight of 54 kD. |
|---|
| Distribution | Ubiquitously expressed/ Nucleus |
|---|
| Function | Transcription factor |
|---|
| Cell Type | Endothelial cells |
|---|
| Biology Area | Cell Biology, Ubiquitin/Protein Degradation |
|---|
| Antigen References | 1. Adamo P, et al. 2016. J. Biol. Chem. 35:4013 2. Birdsey G, et al. 2015. Dev. Cell. 32:82 3. Shah A, et al. 2016. Vascul. Pharmacol. 86:3 |
|---|
| Gene ID | 2078 |
|---|
| UniProt | View information about ERG on UniProt.org |
|---|
| Clone | W19256A |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | ETS-Related Gene, Transcriptional Regulator ERG, ETS Transcription Factor ERG, Erythroblast Transformation-Specific Transcription Factor ERG Variant 10, p55 |
|---|
| Isotype | Rat IgG2a, κ |
|---|
