Biolegend, 947206, Alexa Fluor® 647 anti-FOXO1 Antibody

Forkhead box protein O1 (FOXO1) is a member of the Forkhead box class O family of transcription factors. As such, it is involved in the regulation of a wide range of vital cellular processes including cell cycle progression, differentiation, proliferation, DNA repair, stress response, and apoptosis. FOXO1 is activated via serine and threonine phosphorylation. Phosphorylation is triggered by external stimuli including growth factors, cytokines, and insulin and can be PKB/Akt-mediated as part of the P13K-Akt/SGK pathway on Thr24, Ser256, and Ser319, mediated by CDK2 or CK1 on Ser249 and Ser322/325, respectively, or induced by serum and glucocorticoid-inducible kinase or NFkB kinase. Upon phosphorylation, FOXO1, which is localized to the nucleus, translocates to the cytoplasm following binding to 14-3-3 proteins. In addition to phosphorylation, FOXO1 can be activated via acetylation of its lysine residues by coactivator p300. FOXO1 is vital to the regulation of metabolism through the promotion of glucose homeostasis and gluconeogenic enzyme expression, adipose tissue and skeletal muscle formation, and oxidative stress regulation. FOXO1 may inhibit cell cycle progression and induce apoptosis in cancer cells.