Cytiva, 17009010, Sephadex LH-20, 25 g

Name: Sephadex LH-20, 25 g
Brand: Cytiva
SKU: 17009010

Catalog Number: 17009010

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Category

Process-Scale Chromatography Resins

Pack Size25 g

TagsChromatography resins Size exclusion resins Chromatography products resin

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

Sephadex LH-20 resin is used for preparative purification of natural products such as steroids, terpenoids, lipids, and low molecular weight peptides.
Broad stability in most aqueous mixtures and polar organic solvents.
Powerful chromatographic selectivity from the matrix’s dual hydrophilic/lipophilic nature.
High chemical and physical robustness.
High reproducibility from batch to batch.
Sephadex LH-20 is a resin specifically designed for molecular sizing of natural products in organic solvents.
Sephadex LH-20 resin for organic solvent use
This chromatography resin is made from cross-linked Sephadex G-25 dextran beads, hydroxypropylated to yield both hydrophilic and lipophilic characteristics.
This resin is useful for molecular species preparation at different scales. It can be used during purification, prior to high performance ion exchange or reversed phase chromatography (RPC), or as a final polishing step during diastereomer preparation.
Successful use in published research
Applications of Sephadex LH-20 resin include lipid fractionation and purifying complex organic substances, such as traditional Chinese medicine (TCM), for research use. Examples of successful research use include:
Antioxidant activity in the stem bark of acacia confusa
Free radical scavenging activity in floral corn pollen and pollen found in apis mellifera hives
Antioxidant activity in pinus densiflora needles

Specifications

General

Application	For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures
Autoclavable	20 min at 121°C
Avoid Using	Not stable below pH 2 nor to strong oxidising agents
Chemical stability	Stable in most aqueous and organic eluent systems.
Estimated shelf life from manufacture date	8 years
Exclusion Limit	4 x 10³ (for Peptides) 5 x 10³ (small organics)
Exclusion Limit Peptides	4 x 10³
Exclusion Limit Small Organics	5 x 10³
Flow velocity	< 60 cm/hour
Fractionation range (M r ) globular proteins	4 × 10³ (E×clusion limit will depend on the solvent)–5 × 10³ (E×clusion limit will depend on the solvent)
Matrix	Hydroxypropylated, cross-linked dextran
PSD dry (within specification)	18 µm-111 µm
Particle Size in Methanol Max.	163
Particle Size in Methanol Min.	27
Pressure/Flow Specification	25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.

Ordering

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