| BioProcess resin | Yes |
|---|---|
| Certificate of Analysis | Yes |
| Chemical stability | Stable in commonly used aqueous buffers for size exclusion chromatography, 1.0 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile |
| Fractionation (Mp) Dextrans | 1 × 10³-1 × 10⁵ |
| Fractionation range (M r ) globular proteins | 1 × 10⁴–6 × 10⁵ |
| Matrix | Composite of cross-linked agarose and dextran |
| Particle Size | 24-44 µm |
| Particle size, d 50V | ~34 µm |
| Storage | 4 to 30°C, 20% Ethanol |
| pH stability, CIP | 1–14 |
| pH stability, operational | 3–12 |
Answer:The typical fractionation range is approximately 10,000 to 600,000 Da for globular proteins.
Answer:Size-exclusion chromatography separates molecules by their access to pores in the resin matrix; larger species generally elute earlier than smaller ones.
Answer:No. It is bulk chromatography resin intended for packing an appropriate preparative column.
Superdex 200 Prep Grade is a preparative size-exclusion chromatography medium for separating proteins and protein complexes on the basis of their effective hydrodynamic size. Its composite cross-linked agarose and dextran matrix provides a fractionation range of approximately 10,000–600,000 Da for globular proteins. The 150 mL resin pack enables preparation of columns for aggregate separation, buffer exchange-related fractionation and final protein polishing where molecular size is the principal separation mechanism. For effective resolution, pack a uniform bed, use a compatible isocratic mobile phase and keep injection volume appropriate to column geometry. Sample concentration, viscosity and interactions with the matrix can influence peak shape and recovery. Size-exclusion retention is not a direct measurement of molecular weight without calibration, and resin volume alone does not specify the size of a finished chromatography column.
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