Cytiva, 17531805, Ni Sepharose 6 Fast Flow, 5 L

Name: Ni Sepharose 6 Fast Flow, 5 L
Brand: Cytiva
SKU: 17531805

Catalog Number: 17531805

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Category

Affinity Tag Purification Resins

Pack Size5 L

TagsChromatography resins Chromatography products Affinity resins - tagged proteins resin

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

Ni Sepharose 6 Fast Flow is a high binding-capacity IMAC chromatography resin for purifying his-tagged proteins. Good choice for purification at a variety of scales.
In process scale applications: This resin is used to purify histidine-tagged (his-tagged) and untagged recombinant proteins even at large scales.
In research scale applications: This resin binds 40 mg his-tag protein/mL resin and is suitable for use in batch mode, with gravity flow, or when scalability is needed.
Meets industrial demands with security of supply, robust performance, and regulatory support.
Available as bulk resin and as prepacked columns for use with manual methods or a chromatography system.
Excellent flow properties provide great performance even at high flow rates.
High binding capacity resin with excellent flow properties
Ni Sepharose 6 Fast Flow (FF) is composed of cross-linked 6% agarose beads modified with a chelating ligand immobilized to the base matrix and precharged with nickel ions.
The high dynamic binding capacity combined with the ability to withstand high flow rates gives an overall advantage of being able to increase flow rate and reduce the total time for protein purification considerably with a minimal loss in recovery. Therefore, this immobilized metal affinity chromatography (IMAC) purification resin is a good choice for scaling up.
Successful use in research-scale applications
Life science professionals use Ni Sepharose 6 Fast Flow for his-tag protein purification:
Customer feedback on use in batch purification
IMAC chromatography of human β2-microglobulin in E. coli to 95% purity
Scale-up purification of a viral capsid protein from a prepacked column
Compatibile empty columns – research scale
Cytiva offers a variety of empty columns for packing your own columns. Ni Sepharose 6 Fast Flow is compatible for use with empty PD gravity flow, HiScale, Tricorn, and XK columns.
Compatible empty columns – bioprocess scale
For process-scale applications, Cytiva recommends AxiChrom columns. Ni Sepharose 6 Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.
Prepacked formats – research scale
Ni Sepharose 6 Fast Flow resin is also available in the following prepacked formats:
HisGraviTrap gravity flow column: for simple purification without the need for equipment
His MultiTrap FF 96-well plate: for high throughput screening and small scale purification using centrifuge or vacuum equipment
HisTrap FF crude: HiTrap column format for convenient use with a syringe, a peristaltic pump or a chromatography system.
HiScreen Ni FF column for method and process development
HisPrep FF column for convenient scale-up
Prepacked formats – bioprocess scale
Prepacked ReadytoProcess columns for bioprocess applications are available on request, eliminating the need for column packing and cleaning validation.
Other IMAC chromatography resins and columns
Life Sciences solutions from Cytiva include a variety of nickel-charged resins and other IMAC products. Options for high purity, high resolution, direct loading of cell culture supernatants, and loading of unclarified E. coli lysates are available.

Specifications

General

Chromatography technique	Histidine-tagged protein purification
Binding Capacity/ml Chromatography Medium	Approx. 40 mg histidine-tagged protein
BioProcess resin	Yes
Chemical stability	Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea
Licensing	For licensing information, see the Licensing Statements page in the About Us section.
Matrix	6% cross-linked agarose
Metal Ion Capacity	~15 µmol Ni 2+ /ml medium
Particle Size	45 µm-165 µm
Particle size, d 50V	~90 µm
Pressure/Flow Specification	Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
Storage	4 to 30°C, 20% Ethanol
pH stability, CIP	2–14
pH stability, operational	3–12

FAQs

Question: Which proteins are primarily purified with Ni Sepharose 6 Fast Flow?

Answer:Proteins with accessible polyhistidine tags can bind to its immobilized nickel affinity ligand.

Question: Is this a prepacked HisTrap column?

Answer:No. This item is a 5 L bulk resin pack for packing suitable chromatography columns.

Question: How are many His-tagged proteins eluted from nickel-affinity resin?

Answer:Imidazole-containing elution buffers can compete with histidine-tag interactions, with concentrations optimized for the target.

Application Note

Cytiva Ni Sepharose 6 Fast Flow is a nickel-charged immobilized metal affinity chromatography resin for purification of proteins carrying an accessible polyhistidine affinity tag. The agarose-based medium enables selective binding of histidine-tagged products under suitable neutral-to-mildly alkaline buffer conditions, followed by controlled elution commonly using imidazole. The 5 L bulk resin pack is intended for packing compatible chromatography columns rather than serving as a prepacked disposable column. Optimize binding and wash conditions to remove host-cell proteins and other impurities while maintaining target recovery. Competing chelators and certain reducing conditions may interfere with metal-mediated binding, so check buffer compatibility and target stability before scaling up. Clarify feed material to reduce fouling, and validate column packing, loading capacity and breakthrough behavior for the particular product. After use, follow resin-specific cleaning, regeneration and storage instructions to limit contamination and metal loss. This nickel-affinity resin is not equivalent to protein A or an ion-exchange medium.

Ordering

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Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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