Cytiva, 17543202, Butyl Sepharose High Performance, 200 mL

Name: Butyl Sepharose High Performance, 200 mL
Brand: Cytiva
SKU: 17543202

Catalog Number: 17543202

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Category

Hydrophobic Interaction Resins

Pack Size200 mL

TagsChromatography resins Chromatography products Hydrophobic interaction resins resin

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

Butyl Sepharose High Performance is an aliphatic hydrophobic interaction chromatography (HIC) medium, designed for intermediate and polishing step purification steps when high resolution has priority
Aliphatic HIC resin designed for intermediate purification and polishing steps
Selectivity similar to Butyl Sepharose 4 Fast Flow but higher resolution due to the smaller 34 µm bead size
Relatively high ligand density for high dynamic binding capacity even at relatively low salt concentrations
BioProcess resin supported for industrial applications
The hydrophilic nature of the base matrix ensures low levels of non-specific binding.
Butyl Sepharose High Performance is a member of the Cytiva range of HIC resins for intermediate purification and polishing of proteins. Butyl Sepharose High Performance is based on highly crosslinked, 34-µm agarose beads modified with aliphatic butyl groups via uncharged, chemically stable ether linkages. It is a truly hydrophobic resin displaying a minimum of ionic interactions. Butyl Sepharose High Performance resins is particularly well suited for intermediate purification and polishing steps providing high resolution due to the small particle size.
Butyl Sepharose High Performance is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess resins range, Butyl Sepharose High Performance meets industrial demands with security of supply and comprehensive technical and regulatory support

Specifications

General

BioProcess resin	Yes
Chemical stability	Stable in commonly used aqueous buffers - 1 M sodium hydroxide, 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% acetonitrile, 70% ethanol, 3 M ammoniumsulfate, 1 mM HCl, 30% isopropanol, 2% SDS
Ligand	Butyl
Ligand density	Approx. 50 µmol butyl/ml gel
Matrix	cross-linked agarose
Particle Size	24 µm-44 µm
Particle size, d 50V	~34 µm
Pressure/Flow Specification	Base matrix: 100-200 cm/h, 300 kPa, BioPilot 60/600 column, bed height 30 cm
Storage	4 to 30°C, 20% Ethanol
pH stability, CIP	2–14
pH stability, operational	3–13

Ordering

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Unless otherwise stated, the minimum order amount is USD 1,000.
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