Cytiva, 17543203, Butyl Sepharose High Performance, 1 L

Name: Butyl Sepharose High Performance, 1 L
Brand: Cytiva
SKU: 17543203

Catalog Number: 17543203

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Category

Hydrophobic Interaction Resins

Pack Size1 L

TagsChromatography resins Chromatography products Hydrophobic interaction resins resin

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

Butyl Sepharose High Performance is an aliphatic hydrophobic interaction chromatography (HIC) medium, designed for intermediate and polishing step purification steps when high resolution has priority
Aliphatic HIC resin designed for intermediate purification and polishing steps
Selectivity similar to Butyl Sepharose 4 Fast Flow but higher resolution due to the smaller 34 µm bead size
Relatively high ligand density for high dynamic binding capacity even at relatively low salt concentrations
BioProcess resin supported for industrial applications
The hydrophilic nature of the base matrix ensures low levels of non-specific binding.
Butyl Sepharose High Performance is a member of the Cytiva range of HIC resins for intermediate purification and polishing of proteins. Butyl Sepharose High Performance is based on highly crosslinked, 34-µm agarose beads modified with aliphatic butyl groups via uncharged, chemically stable ether linkages. It is a truly hydrophobic resin displaying a minimum of ionic interactions. Butyl Sepharose High Performance resins is particularly well suited for intermediate purification and polishing steps providing high resolution due to the small particle size.
Butyl Sepharose High Performance is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess resins range, Butyl Sepharose High Performance meets industrial demands with security of supply and comprehensive technical and regulatory support

Specifications

General

BioProcess resin	Yes
Chemical stability	Stable in commonly used aqueous buffers - 1 M sodium hydroxide, 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% acetonitrile, 70% ethanol, 3 M ammoniumsulfate, 1 mM HCl, 30% isopropanol, 2% SDS
Ligand	Butyl
Ligand density	Approx. 50 µmol butyl/ml gel
Matrix	cross-linked agarose
Particle Size	24 µm-44 µm
Particle size, d 50V	~34 µm
Pressure/Flow Specification	Base matrix: 100-200 cm/h, 300 kPa, BioPilot 60/600 column, bed height 30 cm
Storage	4 to 30°C, 20% Ethanol
pH stability, CIP	2–14
pH stability, operational	3–13

FAQs

Question: Which chromatography mechanism does this resin use?

Answer:Hydrophobic interaction chromatography with butyl ligands on a cross-linked agarose matrix.

Question: What is the supplied quantity?

Answer:One liter of bulk chromatography medium.

Question: How is protein elution typically achieved?

Answer:By decreasing the salt concentration after high-salt binding.

Application Note

Butyl Sepharose High Performance is a hydrophobic interaction chromatography medium for the purification and polishing of biomolecules based on surface hydrophobicity. Butyl ligands are coupled to a highly cross-linked agarose matrix to support protein capture or resolution under high-salt binding conditions followed by decreasing-salt elution. The High Performance bead structure enables high-resolution HIC separations in packed chromatography columns. The 1 L bulk resin format is used in method development, scale-up, and production purification where protein hydrophobicity provides useful selectivity after preceding chromatographic steps. It is a chromatography medium, not a ready-to-run prepacked column.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
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