Cytiva, 17543204, Butyl Sepharose High Performance, 5 L
Catalog Number: 17543204
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Category
Pack Size5 L
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| BioProcess resin | Yes |
|---|---|
| Chemical stability | Stable in commonly used aqueous buffers - 1 M sodium hydroxide, 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% acetonitrile, 70% ethanol, 3 M ammoniumsulfate, 1 mM HCl, 30% isopropanol, 2% SDS |
| Ligand | Butyl |
| Ligand density | Approx. 50 µmol butyl/ml gel |
| Matrix | cross-linked agarose |
| Particle Size | 24 µm-44 µm |
| Particle size, d 50V | ~34 µm |
| Pressure/Flow Specification | Base matrix: 100-200 cm/h, 300 kPa, BioPilot 60/600 column, bed height 30 cm |
| Storage | 4 to 30°C, 20% Ethanol |
| pH stability, CIP | 2–14 |
| pH stability, operational | 3–13 |
Answer:5 L of Butyl Sepharose High Performance chromatography resin.
Answer:Hydrophobic interaction chromatography using immobilized butyl groups.
Answer:Protein binding is promoted by suitable high-salt conditions, and elution generally follows a reduction in salt concentration.
Cytiva Butyl Sepharose High Performance is a hydrophobic interaction chromatography (HIC) medium used to separate proteins based on differences in surface hydrophobicity. Butyl ligands are attached to a highly cross-linked agarose-based matrix, enabling protein adsorption under suitable high-salt conditions and release as the salt concentration is reduced. The high-performance resin format supports preparative protein purification and polishing where hydrophobic selectivity complements ion exchange or affinity chromatography. This product supplies 5 L of bulk medium for packing compatible columns in process development and production-scale workflows. It is useful for separating protein variants, aggregates and other components when their hydrophobic interactions differ under the selected process conditions. Butyl Sepharose High Performance is an unpacked chromatography medium rather than a ready-to-run column.
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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