Cytiva, 17547404, MabSelect SuRe LX protein A resin, 5 L

Name: MabSelect SuRe LX protein A resin, 5 L
Brand: Cytiva
SKU: 17547404

A protein A affinity resin developed for high titer antibody processes

Catalog Number: 17547404

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Category

Process-Scale Chromatography Resins

Pack Size5 L

TagsChromatography resins Chromatography products Affinity antibody resin

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

MabSelect SuRe™ LX is a protein A affinity resin with very high dynamic binding capacity at extended residence times. The chromatography resin is developed for processing of monoclonal antibodies at high titers.
Novel, alkali-tolerant rProtein A ligand withstands rigorous clean-in-place and sanitization procedures with 0.1 to 0.5 M NaOH.
High dynamic binding capacity (DBC) for high titer cultures reduces process time and amount of resin used.
Fulfills industrial demands for security of supply , robust performance, and regulatory support .
Excellent for antibody purification in bioprocess applications
Alkali tolerance, high capacity and low ligand leakage in combination with the rigid base matrix makes MabSelect SuRe LX an excellent choice for purification of monoclonal antibodies for clinical applications.
Part of the MabSelect protein A resin family
MabSelect SuRe LX is a member of the MabSelect family of protein A resins for the capture of monoclonal antibodies at process scale. MabSelect SuRe LX shares features and benefits of MabSelect and MabSelect SuRe such as a rigid, high-flow agarose matrix that ensures excellent pressure/flow properties, low non-specific binding that leads to low impurity levels in the eluate pool, and an oriented ligand coupling for excellent binding capacity. It also features the same alkali-tolerant rProtein A ligand as MabSelect SuRe. This protein ligand provides greater stability than conventional protein A-based resins in the alkaline conditions used in cleaning-in-place (CIP) and sanitization protocols.
Enhanced stability of the protein ligand
The enhanced alkali stability of MabSelect SuRe LX improves process economy and product quality; cleaning can be performed with cost-effective reagents such as sodium hydroxide, eliminating the need for expensive and hazardous cleaning agents such as Gua-HCl. Process economy is also improved by the increased dynamic binding capacity of MabSelect SuRe LX.
Just as for MabSelect SuRe, the novel ligand construct of MabSelect SuRe LX has been proven to have an increased stability towards proteases. Therefore, the level of leakage of the MabSelect SuRe LX ligand during elution is low.
Purification platform design advantage
MabSelect SuRe has been demonstrated to give generic elution conditions for different monoclonal antibodies, which can be applied also to MabSelect SuRe LX as it utilizes the same ligand. This is an advantage when designing generic purification platform processes. The feature is also correlated to the construction of the MabSelect SuRe protein ligand.
Prepacked columns and predictor filter plates.
MabSelect SuRe LX is available in the following formats:
Process development scale – prepacked columns and plates
PreDictor MabSelect SuRe LX – 96-well plates for high-throughput screening or process development.
PreDictor RoboColumn MabSelect Sure LX – miniaturized column format for high-throughput process development.
HiScreen MabSelect SuRe LX columns – optimized for method and process development.
Lab/research scale – prepacked columns for manual protein purification
HiTrap MabSelect SuRe LX chromatography columns – for easy use with a syringe, peristaltic pump, or chromatography system.
Lab/research scale – prepacked columns for automated protein purification and analysis
HiTrap MabSelect SuRe LX chromatography columns – for easy use with a syringe, peristaltic pump, or chromatography system.
Process scale protein purification – prepacked columns
ReadyToProcess MabSelect SuRe LX columns – 1 L and larger chromatography columns for clinical manufacturing that avoid the need for cleaning and cleaning validation.
For samples, contact your local sales representative or customer support .
MabSelect SuRe LX protein A resin builds on the proven track record of MabSelect and MabSelect SuRe resins in downstream processing of monoclonal antibodies.
Learn about prepacked HiScreen MabSelect SuRe LX , ReadyToProcess MabSelect SuRe LX columns , PreDictor MabSelect SuRe LX or PreDictor Robocolumns MabSelect SuRe LX

Specifications

General

Chromatography technique	Antibody affinity chromatography
Binding Capacity/ml Chromatography Medium	Approximately 60 mg human IgG/mL resin.
BioProcess resin	Yes
Certificate of Analysis	Yes
Chemical stability	Stable in all aqueous buffers commonly used in Protein A Chromatography
Ligand	Alkali-stabilized Protein A-derived ( E. coli )
Ligand Coupling Method	Epoxy activation
Matrix	Highly cross-linked agarose
Particle size, d 50V	~85 µm
Pressure/Flow Specification	500 cm/h, AxiChrom 300, bed height 20 cm, operating pressure < 2 bar
Storage	2 to 8°C, 20% Ethanol
pH stability, CIP	2–13.7
pH stability, operational	3–12

FAQs

Question: What purification step uses MabSelect SuRe LX?

Answer:Protein A affinity capture of monoclonal antibodies or other proteins with a compatible IgG Fc region.

Question: How much resin is supplied in this configuration?

Answer:Five liters of MabSelect SuRe LX resin, suitable for larger column-packing and capture-process requirements.

Question: Can the resin undergo alkaline cleaning?

Answer:Yes. Validated cleaning-in-place procedures can use 0.1–0.5 M NaOH, with exposure conditions established for the process.

Application Note

Cytiva MabSelect SuRe LX Protein A resin is supplied in a 5 L package for antibody-capture chromatography processes that require larger resin inventories. Its cross-linked agarose matrix carries an alkali-stabilized protein A-derived ligand that binds the Fc region of human IgG, enabling high-selectivity capture before subsequent polishing steps. Dynamic binding capacity is approximately 60 mg human IgG per mL of resin at a six-minute residence time under defined test conditions, with actual capacity varying by antibody, buffer and operating conditions. The resin tolerates validated alkaline cleaning with 0.1–0.5 M NaOH and is supplied in an ethanol-containing storage solution. The larger pack can supply columns for pilot or manufacturing process operations and facilitates preparation of multiple columns using a common medium. Column packing, breakthrough targets, cleaning exposure and resin lifetime require process-specific verification.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
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