Cytiva, 17547703, VIISelect, 1l
Catalog Number: 17547703
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Pack Size1 L
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| Chromatography technique | Group specific affinity |
|---|---|
| BioProcess resin | No |
| Ligand | Recombinant protein (M r 14 080) produced in Saccharomyces cerevisiae |
| Ligand density | Approx. 5.7 mg/ml of medium |
| Matrix | Highly cross-linked, high-flow agarose |
| Particle size, d 50V | ~75 µm |
| Pressure/Flow Specification | At least 600 cm/h in a 1 m column with 20 cm bed height at 20°C using process buffers with the same viscosity as water at < 3bar (0.3 Mpa) |
| pH stability, CIP | 2–12 |
| pH stability, operational | 3–10 |
Answer:VIISelect is an affinity resin developed for the capture and purification of coagulation factor VII.
Answer:The bulk package contains 1 L of VIISelect chromatography resin.
Answer:No. Cytiva's VIISelect literature explicitly excludes use for purification of transgenic factor VII.
Cytiva VIISelect is a specialized affinity chromatography resin designed for purification of coagulation factor VII and factor VII-related products using selective ligand interactions. The resin supports a defined capture step in an appropriately developed protein purification process rather than general histidine-tag or protein A binding. This 1 L bulk format is suitable for packing compatible columns and evaluating process-scale capture performance. Establish binding and elution conditions around the specific factor VII molecular form, feed composition and stability profile, and evaluate breakthrough, recovery, activity and impurity clearance before scale-up. Use appropriate equilibration and washing buffers and maintain validated cleaning and storage procedures to preserve ligand performance. Specific process claims depend on feedstock, loading conditions and the required regulatory controls. The product is not intended as a generic blood-clotting assay reagent or as a ready-to-run prepacked column. Confirm the intended source and process qualification scope because VIISelect is not offered for purification of transgenic factor VII.
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