Cytiva, 17550102, MabSelect PrismA™ X protein A resin, 200 mL

Name: MabSelect PrismA™ X protein A resin, 200 mL
Brand: Cytiva
SKU: 17550102

Protein A chromatography resin with excellent capacity and alkaline stability for cost-efficient and extremely robust mAb capture.

Catalog Number: 17550102

Pricing

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Category

Process-Scale Chromatography Resins

Pack Size200 mL

TagsChromatography resins Prepacked columns Chromatography products Affinity antibody resin

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

MabSelect PrismA™ X protein A resin is a high-capacity chromatography resin designed to efficiently capture monoclonal antibodies (mAbs). It features exceptional alkaline stability for repeated cleaning in place (CIP) to reduce bioburden.
Dynamic binding capacity (DBC) higher than 80 g of mAb/L resin at 6 min residence time (RT), saving on resin and buffer consumption.
Excellent alkaline stability enables efficient cleaning and sanitization using 0.5 to 1.0 M NaOH for improved process economy and robustness.
Well-established protein A ligand used with several chromatography matrices. Supported by the PrismA ELISA kit for accurate and convenient detection of leached protein A ligand.
Designed for use in short bed height applications using rapid cycling chromatography (RCC) for improved productivity in the protein A capture step.
DBC at different residence times
The resin is based on a highly cross-linked agarose base matrix with a median particle (bead) size d 50v of 50 μm. The DBC for MabSelect PrismA™ X resin using trastuzumab is excellent. At 6 min, the DBC is 82 mg/mL for MabSelect PrismA™ X resin and 74 mg/mL for MabSelect PrismA™ resin. As the high DBC is realized at short RT, the MabSelect PrismA™ X resin is beneficial for use in RCC to further reduce resin volumes to increase grams of mAb produced per liter of resin and decrease overall resin costs.
Alkaline-stable protein A ligand
We developed the ligand used in MabSelect PrismA™ X and MabSelect PrismA™ resins to withstand high concentrations of NaOH. The enhanced alkaline stability lets you perform efficient cleaning of the resin using 0.5 to 1.0 M NaOH over many purification cycles, providing long resin lifetime and minimized risk for bioburden incidents.
Formats
MabSelect PrismA™ X resin is available as bulk resin in 25 mL, 200 mL, 1 L, 5 L, and 10 L containers. The resin can be ordered in prepacked column formats such as HiTrap™ columns (1 and 5 mL), HiScreen™ columns, and on request as production-scale ReadyToProcess™ columns. These formats enable use from lab-scale to commercial-scale manufacturing.
Cytiva’s mAb toolbox
Process volumes, number of batches, target molecule, impurity profile, and cleaning protocols influence which chromatography resin will be most cost-efficient and productive in a given application. We can support you in a discussion about how to set up your process and to make your process more cost efficient. And with every resin you choose, you have Cytiva quality, an extensive security of supply program, and access to our product knowledge to go along with it. Contact us for a productivity discussion .

Specifications

General

Chromatography technique	Antibody affinity chromatography
BioProcess resin	Yes
Certificate of Analysis	Yes
Dynamic binding capacity, Q B10	> 80 mg mAb/mL resin at 6 min residence time, > 74 mg mAb /mL resin at 4 min residence time
Ligand	Alkaline-stable protein A-derived ( E. coli )
Matrix	Highly cross-linked agarose
Particle Size	50 µm
Recommended maximum operational flow velocity	< 220 cm/h (3 bar [43.5 psi, 0.3 MPa], 20 cm bed height), < 400 cm/h (3 bar, 10 cm bed height)
pH stability, CIP	0.5 to 1.0 NaOH
pH stability, operational	3 to 12

FAQs

Question: What is the intended capture target?

Answer:Many antibodies and Fc-containing proteins that bind a protein A affinity ligand.

Question: How much resin is supplied?

Answer:A 200 mL pack of bulk MabSelect PrismA X protein A chromatography resin.

Question: Is it a ready-to-use prepacked column?

Answer:No. It is a bulk medium that requires compatible column hardware and a qualified packing procedure.

Application Note

Cytiva MabSelect PrismA X is a protein A affinity chromatography resin offered in a 200 mL bulk-resin pack for antibody capture and process development. The protein A ligand provides selective binding to the Fc regions of appropriate immunoglobulins, making the medium useful for purification of many monoclonal antibodies from clarified cell culture harvest. High-capacity affinity capture can reduce downstream load, although the usable capacity and recovery must be established for each antibody and loading residence time. Equilibrate the packed bed with a suitable binding buffer, load clarified harvest, wash impurities away and elute the captured antibody under a validated low-pH or alternative elution protocol. Control exposure to elution conditions to reduce aggregation or loss of biological activity. Verify packing quality and pressure–flow performance before process studies, and qualify the cleaning and storage strategy for the intended number of cycles. The supplied material is unfilled resin for packing, not a prepacked HiTrap or HiScreen column.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

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