Description
Trends in rolling circle amplification: Capabilities for manipulation, error free production and assembly of nucleic acids are advancing. Find out how TempliPhi™ amplification kits use isothermal rolling circle amplification (RCA), enabled by phi29 DNA polymerase, to support these advancements . One-tube, one-temperature format TempliPhi™ kits use a unique kind of circular DNA amplification to efficiently prepare micrograms of DNA from picogram circular DNA starting material. TempliPhi™ kits use isothermal rolling circle amplification (RCA) for the exponential amplification of circular DNA using bacteriophage Phi29 DNA polymerase. The amplification used is often called multiple strand displacement amplification, or MDA. Phi29 DNA polymerase is active at 30°C, and isothermal amplification is performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol allows amplification of 96 samples from bacterial colonies with less than 20 min of hands-on time. Two different types of TempliPhi™ DNA Amplification Kits are available to meet different throughput requirements. TempliPhi™ DNA Amplification Kit for 2000 reactions, designed for automation, contains pre-mixed components and requires overnight incubation. TempliPhi™ 100 and TempliPhi™ 500 reaction kits contain separate, unmixed components for enhanced stability and preparations are ready in 4 to 6 hours.
