Cytiva, 27209002, 7-Deaza-2'-Deoxyguanosine 5'-Triphosphate, 5 mM solution (c⁷dGTP)

Name: 7-Deaza-2'-Deoxyguanosine 5'-Triphosphate, 5 mM solution (c⁷dGTP)
Brand: Cytiva
SKU: 27209002

Nucleotide analog (candidate substrate) for processive telomerase, to improve PCR yields and make sequencing of GC rich sequences easier.

Catalog Number: 27209002

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Category

Diagnostic ELISA Kits

TagsMolecular and Immunodiagnostics Western blotting and other genomics reagents

CollectionsCytiva Molecular & Immunodiagnostics Collection Cytiva Protein Analysis Equipment & Supplies Collection

Description

Deaza dGTP is a substrate for most DNA polymerases, including Taq DNA polymerase . It can also be applied to dideoxy-chain termination sequencing methods, in place of dGTP to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA. Partial substitution of Deaza dGTP for dGTP in PCR can improve the product yield when the template is GC-rich. Technical Information: *Band compressions are ambiguous band patterns on DNA sequencing gels. This is caused by stable, intra-strand secondary structures which are not fully denatured during electrophoresis. The likelihood of occurrence can be reduced through substitution of nucleotide analogs within sequencing mixers. To resolve G-band compressions, use 7-deaza dGTP (c⁷dGTP).

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