Cytiva, RPN2108, Amersham™ ECL™ Western blot detection kit, for detecting 1000 cm²

Amersham ECL Western Blotting Detection Kit uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL Detection Reagents results in a chemiluminescent signal that can be captured on film or detected with a CCD camera. ECL Western Blotting System is the world’s most widely used and referenced chemiluminescent immunodetection system. Each system contains the following reagents, sufficient for detection of 1000 cm² membrane: Anti-mouse HRP conjugate, 100 µl Anti-rabbit HRP conjugate, 100 µl ECL Detection Reagents 1 and 2, 62.5 ml each Blocking agent, 5 g.