Cytiva, SH30005.04, HyClone™ IMDM powder with L-glutamine, HEPES, without alpha-thioglycerol, 50 L

Cytiva HyClone Iscove's Modified Dulbecco's Medium (IMDM) powder is a dry basal medium for preparing 50 L of mammalian cell-culture medium after reconstitution. The formulation includes L-glutamine and HEPES buffering agent but excludes alpha-thioglycerol. IMDM supplies inorganic salts, amino acids, vitamins, glucose, and other nutrients used to support selected mammalian cell culture protocols. Its powdered format is useful when laboratories prepare larger batches of basal medium rather than purchasing ready-to-use liquid. Reconstitution requires controlled water quality, adjustment of pH, and addition of sodium bicarbonate following the IMDM preparation instructions before sterilizing the final solution by an appropriate validated method. Serum or other supplements can be added separately as required by the chosen cell line and culture conditions.