Cytiva, SH30859.02, HyClone CDM4HEK293 powdered medium without phenol red, L-glutamine, HEPES, sodium bicarbonate - 10 L

Cytiva HyClone CDM4HEK293 powdered medium supports suspension culture of HEK293 cells used in recombinant protein and viral-vector manufacturing. The chemically defined formulation is protein-free and free of animal-derived components, supporting controlled upstream-process development and consistent medium preparation. This powdered 10 L configuration excludes phenol red, L-glutamine, HEPES and sodium bicarbonate, allowing the cultivation process to specify and add those components separately when needed. Reconstituted medium can be incorporated into seed-train expansion or production stages after adjustment of concentration, pH, osmolality and appropriate filtration. HEK293 cell adaptation, feeding strategy and culture performance should be assessed using the laboratory's specific cell line and production objective. The 10 L unit represents the nominal amount of prepared medium, not a ready-to-use 10 L liquid container. Prepared-medium handling and storage follow the validated manufacturing procedure.