Description
Product Overview The X-VIVO® 10 Media formulations are designed to support the generation of LAK cells in a serum-free environment. The original protocols involved the incubation of patient or normal donor peripheral blood lymphocytes (PBL) at 1.0 – 3.0 × 10 6 cells/ml for a period of 3 days in the presence of 1,000 Cetus units of rIL-2/ml. Optimal LAK cell generation is achieved when peripheral blood lymphocytes are incubated for 3 – 10 days at a density of 1.0 – 6.0 × 10 6 cells/ml in the presence of 100 – 1,000 Cetus units of rIL-2. X-VIVO® 10, catalog number 04-380Q contains L-glutamine, gentamicin, and phenol red. THIS PRODUCT IS NOT FOR USE IN GMP MANUFACTURING, NOR HUMAN OR ANIMAL IN VIVO USE, INCLUDING USE AS A DILUENT OR AS AN EXCIPIENT, OR FOR DIAGNOSTIC USE. This product is for research use only. If you require GMP grade media, contact Lonza for more details. Request More Info
Benefits Superior media for most hematopoietic cells types including dendritic cells, lymphocytes, monocytes, macrophages and granulocytes. Formulations only contain human derived components Highly cited in scientific literature Clinical proven formulations providing process safety and constancy High productivity resulting in a high viable cell yield Serum Free media result in a more defined process and result in limited immune response Efficient transgene expression Desirable T-cell phenotype Applications Proliferation of peripheral blood lymphocytes Proliferation of tumor infiltrating lymphocytes Cryopreservation of human tissue Cultivation of human monocytes and macrophages Cultivation of stem cells Cultivation of dendritic cells
Storage and Content 1L bottle
