Lonza, BE02-059E, Ferric Citrate Agent 100 ml Bottle

Name: Ferric Citrate Agent 100 ml Bottle
Brand: Lonza
SKU: BE02-059E

Size: 100 ml, Ferric Citrate (100x) Agent, 100ml

Catalog Number: BE02-059E

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Category

Media Bottles

Pack Size100 mL

TagsCulture Media and Reagents

Description

Product Overview Chinese Hamster Ovary (CHO) cells are widely used in large-scale culture. These cells have conventionally been grown as attached cultures, but they will also grow in suspension, a property which has allowed for relatively simple scale up in stirred tank and airlift bioreactors. Unfortunately, some CHO-derived lines which have been adapted to suspension growth can form large, tightly bound aggregates in culture. Why is clumping a problem? In batch suspension cultures, cell aggregation hinders accurate cell counting, monitoring and control of the cellular environment Transport of nutrients to, and products from, the cells may be impaired Cluster formation dramatically influences the growth behavior of the cells Cells within aggregates show a strongly reduced proliferation rate Shear forces exerted on large aggregates cause a considerably higher specific death rate than those exerted on single cells, reducing the specific growth rate up to 50 % To solve the problem of cell aggregation, Lonza introduces Anti-clumping Agents A (BE02-058E) and B (BE02-059E, F and Q), two ready-to-use solutions designed to minimize CHO cell clumping in suspension cultures. Both solutions are made of non-animal origin components. Anti-clumping Agent A is a buffered, concentrated solution of Dextran sulfate, and recommended usage is at 100 × dilution Anti-clumping Agent B is a buffered, concentrated solution of ferric citrate, and recommend use it at 100× or 200× dilution As a result of using Anti-clumping Agents from Lonza, clumping of cells cultured in serum-free medium is minimized, leading to a significant increase in cell density and productivity. This BioWhittaker® Cell Culture Media is produced according to cGMP and labelled "For Further Manufacturing" (FFM). GMP Products are intended for research or further manufacturing use only. THIS PRODUCT IS NOT INTENDED FOR DIRECT THERAPEUTIC USE IN HUMANS. If you require different pack sizes, contact Lonza for more details. Request More Info
Benefits Anti-clumping agent to maximize cell growth Easily scaleable to support high-density, large scale production volumes Applications High yield protein production in CHO cells Included in PowerCHO® 2 Powder Kit and Feeds
Storage and Content 100 ml bottle

FAQs

Question: What is the main application?

Answer:It is a ferric-citrate-based anti-clumping supplement for suspension CHO cultures.

Question: What concentration and package are supplied?

Answer:It is supplied as 100 mL of 100× concentrate.

Question: What dilution is used?

Answer:The concentrate can be diluted 100-fold or 200-fold for anti-clumping use.

Application Note

Ferric Citrate Agent 100 ml Bottle is designed for reducing aggregation of Chinese hamster ovary (CHO) cells in suspension cell-culture processes. The non-animal-origin, buffered ferric citrate formulation is supplied as a 100 mL bottle of 100× concentrate. It is used as an anti-clumping supplement where aggregated CHO cells interfere with accurate cell counting, process monitoring or the exchange of nutrients and cell products. The concentrate can be diluted 100-fold or 200-fold for the anti-clumping application. Reducing the formation of large, tightly bound cell aggregates supports more consistent suspension-culture observations and can help preserve a more homogeneous cell environment. This product is the ferric-citrate-based anti-clumping option rather than the separate dextran-sulfate-based agent. It is applicable to suspension CHO culture and process-development workflows in which aggregate formation is a known concern. The precise bottle volume and ferric-citrate composition distinguish this version from other package sizes and alternative anti-clumping formulations.

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