Description
Product Overview Normal and Diseased Bronchial Epithelial (NHBE and DHBE) cells are isolated from epithelial lining of airways above bifurcation of the lungs. Lonza's Normal Human Bronchial Epithelial Cells (NHBE) are available with retinoic acid. All cryopreserved Human Bronchial Epithelial Cells are guaranteed through 15 population doublings. Cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Select Applications with Lonza's Human Bronchial Epithelial Cells - "A Rotating Bioreactor for Scalable Culture and Differentiation of Respiratory Epithelium”. Respiratory epithelium is difficult to grow as it requires a well-maintained polarizing air‐liquid interface (ALI) to maintain differentiation. Raredon et al . describe a scalable and easy method to conduct air-liquid interface with Lonza's bronchial epithelial cells guaranteed for ALI differentiation (Cat. No. CC-2540S). Cell concentration and space for growth are required for productive NHBE cell branching in vitro . Click to read this article by Hagiwara et al. that shows how co-cultures with HUVECs in 3D culture influence reconstruction of branched tubular structures in lung epithelial cells. Wu, et al . reports for the first time that HBE cells when grown in B-ALI TM Medium can differentiate into 3D glandular acinar structures, likesalivary and mammary epithelial. The cells were grown on the basement membrane matrix Matrigel under select conditions and have shown to exhibit formation of brochospheres or tracheospheres with ciliated lining. Purchase our Bronchial Epithelial Cell Culture Kit (catalog no. CC-2540B-available in US only) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of bronchial epithelial cells (catalog no. CC-2540) and BEGM® BulletKit® Growth Media (catalog no. CC-3170) Request More Info
Benefits All cells test negative for mycoplasma, bacteria, yeast, and fungi HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots Certificate of Analysis provided for every cell lot
Storage and Content 1 x cryopreserved ampoule NHBE with retinoic acid containing ≥500,000 cells
