Description
Product Overview Lonzas Peripheral Blood Mononuclear Cells (PBMCs) are isolated from peripheral blood by apheresis and density gradient separation. Cells are collected from healthy donors following IRB protocols. They are guaranteed to contain ≥50 million viable cells per ampoule. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Mononuclear cell (MNC) rich cells from leukapheresis are depleted of RBCs and platelets. MNC cell profiles differ by donors and can contain on average around 50% T cells, 20% monocytes, 10% B cells, 10% NK cells and may contain up to 5% granulocytes. PBMCs of this part code have added characterization information available with population frequency values for T cells (CD3, CD4, CD8), B cells (CD19), Monocytes (CD14), NK cells (CD56), Dendritic cells (CD11c). With this information select the donor best suited to your experimental needs Ability to Screen Different Donors We maintain an inventory of different donors in an effort to satisfy your sample representation requirements. You can choose a lot based on donor demographic characteristics, and screen multiple lots to find the lot(s) that work best for you. In addition, we can request repeat donors if you need additional cells from a particular donor. Rich Source of Immune Cells Peripheral blood mononuclear cells (PBMC) are a mixed population of single nucleus cells. They can be further purified to isolate individual cell types such as natural killer cells, T cells, and B cells. In addition, PMBCs are a rich source of monocytes, which can be directed to differentiate into either macrophages or dendritic cells through culture with various cytokines. Researchers can choose X-Vivo 15 Hematopoeitic media that supports maintenance of lymphocytes and is optimized for serum-free culturing (additional cytokines may be necessary depending on application). Please contact Scientific Support for serum containing media recommendations. Lonza guarantees the performance of Clonetics TM /Poietics TM cells only if appropriate Clonetics TM /Poietics TM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support. Request More Info
Benefits Test negative for bacteria, yeast, and fungi HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots Certificate of Analysis (CoA) is provided for each cell lot purchased Applications T-cell Proliferation assays Cytokine release assays Macrophage polarization assay Immunogenicity studies Cell therapy process development Humanized mice Biomarker discovery Inflammatory studies Immuno-oncology CAR-T research
Storage and Content 1 x Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 50 million cells
